Effects Of Total Flavonoids Of Astragalus On Ros Level And Antioxidant Enzymes Expression In Oxidative Damage Mouse Embryos

In vitro culture of embryo is an important part of in vitro fertilization and embryo transplantation(IVF-ET).However,the lack of high-quality embryos will result in low efficiency of transplantation,which is also an important factor in the failure of IVF-ET.In studies to evaluate,of the various harmful factors that may result in developmental blockage,the effects induced by excessive reactive oxygen species(ROS)are considered to be among the most significant ones.Thus,antioxidants are widely tested in the cultivation of embryos,in order to clear out excessive ROS.In this study,the effect of total flavonoids of astragalus(TFA)on the development,ROS level and the expression of antioxidant enzyme genes in Kunming mouse during in-vitro culture.In the first experiment,effect of different concentration(0 mg/L,25 mg/L,50 mg/L,75 mg/L and 100 mg/L)of TFA was used to determine optimal dose rate.In Subsequent experiments embryos were divided into three groups control,H2O2 treated and optimal concentration TFA+H2O2 treated groups.Our result shows that the 4th cell and blastocyst rate was significantly higher in 75 mg/L TFA group(P<0.05)than the other groups.The intracellular ROS level was significantly higher in H2O2 group(P<0.05)which was significantly decrease after the supplementation of TFA.Moreover the mRNA expression level of SOD1 and SOD2 in the TFA+H2O2 group were significantly higher than the H2O2 group(P<0.05),however the mRNA expression level of CAT,GPxl and GPx4 were significantly higher in TFA+H2O2 group as compare to H2O2 group(P<0.01).In conclusion,the present shows that the optimal dose rate of TFA in vitro embryo culture was 75 mg/L.TFA treatment can reduce ROS level,improve the expression levels of CAT,SOD1,SOD2,GPx1 and GPx4 in blastocyst of mice in oxidative stress condition.