The Effect Of Bete-Asarone On Brain Damage In Rats With Parkinson's Disease

ObjectiveThe effect of ?-asarone on the activity of MEF2 D was investigated by using 6-OHDA model rats,and the mechanism of ?-asarone on the regulation of HSP70 / MAPK / MEF2D/ Beclin-1 pathway was further explored.The patient's treatment provides an important experimental basis.MethedsSuccessfully modeled SD rats were randomly divided into 4 groups: 6-OHDA model group,?-asarone group,MEF2 D inhibitor group,MEF2 D activator group,and compared with the sham operation group.After 30 days of intervention,SD rats were observed by behavioral statistics such as autonomous activity counting;?-syn,TH,LAMP-2A,HSP70 and MEF2D levels were detected by enzyme-linked immunosorbent assay;Beclin-1,LC3 B were detected by immunoblotting.P62,HSP70,HSC70 level expression,The analysis was carried out using SPSS 21.0.The statistical methods mainly include ANOVA and Pearson correlation analysis.Results1 The behavioral observation of ?-asarone on PD model rats: The 6-OHDA model group was significantly lower than the sham operation group in terms of autonomous activity count and balance ability,and was significantly higher in the forelimb start time and forelimb step time than the sham operation group(P <0.05);Compared with the model group,the MEF2 D activator group and the ?-asarone group had significantly increased autonomic activity count and balance ability,and the forelimb start time andforelimb step time decreased(P <0.05);MEF2D The results of the comparison between the activator group and the ?-asarone group showed that there was no significant difference in the autonomous activity count,roller test and forelimb start time and pace time(P>0.05).2 The results of ELISA showed that the level of ?-syn was the lowest in the sham operation group,and the expression levels of TH,LAMP-2A,HSP70 and MEF2 D were the highest.The ?-syn level in the 6-OHDA model group was significantly higher than that in the sham operation group(P <0.01),and TH,LAMP-2A,HSP70 and MEF2 D were significantly lower than the sham operation group(P <0.01).Compared with the 6-OHDA model group,the level of ?-syn was increased in the MEF2 D inhibitor group,and the expression levels of TH,LAMP-2A,HSP70 and MEF2 D were decreased(P < 0.05);MEF2D activator group and ?-asarone group ? The level of-syn decreased,and the expression levels of TH,LAMP-2A,HSP70 and MEF2 D increased(P < 0.05).In addition,the levels of ?-syn in the MEF2 D activator and the ?-asarone group were decreased,and the expressions of TH,LAMP-2A,HSP70 and MEF2 D were increased(P < 0.05)compared with the MEF2 D inhibitor-treated group.3 The results of HSC70,MEF2 D and HSP70 detected by Western Blotting showed that the expression level of MEF2 D inhibitor group decreased,and the expression level of MEF2 D activator group and ?-asarone group increased compared with 6-OHDA model group(P<0.05);Compared with the inhibitor group,the expression levels of MEF2 D activator group and ?-asarone group increased(P<0.05).The expression level of the?-asarone group was lower than that of the MEF2 D activator group(P<0.05).4 Western Blotting detection of Beclin-1,LC3 B and p62 showed that the expression levels of Beclin-1 and LC3 B were decreased in MEF2 D inhibitor group,MEF2 D activator group and ?-asarone group compared with 6-OHDA model group.The level was enhanced(P<0.05);the expression level of Beclin-1 and LC3 B was the lowest in ?-asarone group and the highest in p62(P<0.05).5 Pearson correlation analysis showed that the correlation coefficients between ?-syn,TH,Beclin-1,LC3 B,p62,HSP70,LAMP-2A and MEF2 D levels were-0.516,0.538,-0.567,-0.674,0.571,0.958,respectively.At 0.809,the difference was statistically significant(P < 0.05).6 PCR results showed that the levels of ?-syn and Beclin-1 mRNA were significantly increased in the 6-OHDA model group compared with the sham operation group,and the changes in MEF2 D mRNA levels were opposite(P<0.01);and 6-OHDA Compared with the model group,the ?-syn and Beclin-1 mRNA levels were decreased in the ?-asarone group and the MEF2 D activator group,and the MEF2 D mRNA level was increased.Similarly,the ?-asarone group was compared with the MEF2 D inhibitor group.The levels of ?-syn and Beclin-1 mRNA were decreased in MEF2 D activator group,and the level of MEF2 D mRNA was increased(P<0.01).Compared with MEF2 D activator group,?-syn,Beclin-1 in ?-asarone group And MEF2 D mRNA levels were decreased(P < 0.01).Conclusion1 ?-asarone can improve the autonomic activity and balance ability of PD model rats.2 ?-asarone can reduce ?-syn levels by activating CMA and increase the expression levels of HSP70,MEF2 D,LAMP-2A and p62 in PD model rats,and down-regulate the expression of Beclin-1 and LC3 B.3 ?-asarone can attenuate 6-OHDA induced dopaminergic neuronal damage by modulating the HSP70 / MAPK / MEF2 D / Beclin-1 pathway.