Expression And Analysis Of EPOR After Radiation Injury Of Salivary Glands In Rats

Introduction:Radiation therapy has now developed into a most effective treatment for patients with head and neck malignancies,and has achieved good clinical results.However,because the patient’s salivary glands are on the surface of the tumor cells or directly on the surface of the tumor,most of the time they are in the radiological field of view,and because the salivary glands,especially some submandibular glands or parotid glands rich in serous acinus,are very radioactive.Sensitive,therefore,in patients with head and neck cancer,radiation therapy is often accompanied by complications such as radiation damage to the salivary glands.The erythropoietin receptor(EPOR),a single-stranded transmembrane protein,consists mainly of 484 amino acids[31.According to the study,the nucleotide sequence homology of mouse and human is as high as 81.6%,and the EPOR structure can be divided into three parts,followed by extracellular region,intracellular region and transmembrane region.A total of 226 amino acids,in which the transmembrane region is composed of 22 hydrophobic amino acids,the signal peptide contained in the extracellular portion of the extracellular portion is composed of 24 amino acids[1].The current research results of this research group show that erythropoietin(EPO)can protect against the radiation damage of salivary glands and the substance needs to bind to the EPOR receptor to form a homodimer to effectively exert its efficacy[2].The main reason is that when EPO and EPOR receptors are combined,it will mediate the autophosphorylation of the latter tyrosine kinase JAK-2,which leads to phosphorylation of many downstream signaling pathways,and finally shows protec tive effects.In this study,we established a model of rat submandibular gland radioactive injury to study the expression of erythropoietin receptor,which provides a theoretical basis for further exploration of radioactive salivary gland dysfunction.PurposesThe expression of EPOR in the submandibular gland of rats after radioactive injury was analyzed,which provided theoretical support for further clinical research.Method30 male Wistar rats are divided into a control group and an experimental group.The latter is given radiation treatment(part:neck,irradiation:one-time receiving 15Gy high-dose radiation).Then,on the 3rd,30th and 60th,the submandibular glands of the two groups of subjects were taken for paraffin sectioning,and then HE staining was performed.The expression of the EPOR protein was observed by immunohistochemical staining,and the average optical density value was calculated according to ImagePro Plus 6.0 for quantitative analysis.Results① After the treatment with the reflection line,the EPOR of the rats was significantly increased compared with the control group;② There is a certain correlation between the expression of EPOR in the control group and the age-relatedness of the rats;③ Studies have shown that rat EPOR is positively expressed in the ductal part of the gland,but no acinar cells have been observed.ConclusionWhen the rat salivary gland undergoes radioactive damage,it will mediate an increase in EPOR expression.