Study On The Role And Mechanism Of Mir-199b In Promoting Vascularization Of Chronic Wound

Objective To explore the effects of mir-199 b on proliferation and migration of HaCaT cells,as well as VEGFA,JAG1 and SET signaling pathways.Methods Transfection of mir-199 b mimics and inhibitor lentivirus into human immortalized epidermal cells HaCaT was performed.The study was divided into three groups: mir-199 b up,mir-199 b down and no-load control.CCK8,cycle and scratch test were conducted,and the expression of VEGFA,JAG1 and SET genes were detected by RT-PCR,and the downstream protein changes(such as VEGFA,JAG1 and other molecules)were detected by WB technology.Results The OD450/fold value of mir-199 b up group was significantly higher than that of NC group(P<0.05).The OD450/fold value of mir-199 b down group was slightly lower than that of NC group(P>0.05).In the G1 phase,cells in the mir-199 b up and down group were increased compared to the control group(P<0.05).At the S stage,compared with the control group,the cells in the mir-199 b up and down group were reduced(P>0.05).In the G2/M phase,compared with the control group,the cells in the mir-199 b up and down group were reduced(P>0.05).Compared with the NC group,the percentage of apoptotic cells in the mir-199 b up group decreased(P<0.05)and the percentage of apoptotic cells in the mir-199 b down group increased(P<0.05).The expression of SET was significantly lower in the mir-199 b up group than in the NC group,and significantly higher in the mir-199 b down group than in the NC group.According to the quantitative PCR results,the expression abundance of JAG1 in the mir-199 b up group was slightly higher than that in the NC group(P>0.05).The expression abundance of JAG1 in the mir-199 b down group was significantly higher than that in the NC group(P<0.05).The expression abundance of VEGFA in the mir-199 b up group was significantly lower than that in the NC group(P<0.05).The expression abundance of VEGFA in the mir-199 b down group was significantly higher than that in the NC group(P<0.05).SET expression abundance of mir-199 b up group was smaller than that of NC group(P>0.05).The expression abundance of SET in the mir-199 b down group was significantly greater than that in the NC group(P<0.05).Conclusion mir-199 b is the target gene of VEGF,JAG1 and SET proteins by inhibiting their expression.It can promote cell proliferation,prolong cell cycle and inhibit cell apoptosis.