| Objective Through comparing three different methods of model ulcerative colitis:inactivated escherichia Coli(e.Coli strains)immune + acetic acid(AA)enema,pure inactivated escherichia Coli(e.Coli)immunity,pure acid(AA)enema to explore an ideal method.The treatment mechanism of GXT in rats ulcerative colitis was made in-depth study from colon tissue,blood index,serum immunology index,colon tissue immune index,liver and kidney function,etc.It shows an good prospects for GXT in treatment of ulcerative colitis.Methods 1.One hundred and twenty health SD male rats were randomly divided into four groups.On days 1,7,14,and 20 of the experiment,the suspension of inactivated E.coli strain was injected into the sole,abdomen,back subcutaneous and abdominal cavity of rats in groups 1 and 2.The same amount of normal saline was injected into the rats in group 4 as a control group.On day 21 of the experiment,rats in groups 1 and 3 were enema with 5% acetic acid.The mental state,coat color,diet,and stool characteristics of the rats in each group were observed daily.Rats were weighed weekly and tested for fecal occult blood Ten rats in each group were randomly sacrificed at the 1w,2w,and 3w after acetic acid enema.After decapitated blood was taken,changes in serum IL-6 concentration were measured and the length and weight of the colon were observed.The colonic mucosal tissue changes were observed by naked eye and HE staining,and the positive expression of TNF-? in the colon was detected by immunohistochemistry.2.According to the randomization principle,40 rat UC models treated with(E.Coli)strain +(AA)enema were divided into GXT high dose group,GXT middle dose group,GXT low dose group and control group.The rats inthe high,middle and low dose groups of GXT were administered with 24g/kg,12g/kg and 6g/kg of crude drugs.The mental state,coat color,diet,stool traits and body weight of rats in each group were observed,and the fecal occult blood was detected.,The above 40 rats were sacrificed 21 days after the gavage treatment.Serum IL-6,IL-10,leukocytes,platelets,SCr,BUN,AST,ALT were measured,and colon length and weight were measured.The histological changes of the intestinal wall were observed,and the expression of TNF-? and i NOS in the colon was detected by immunohistochemistry.Results 1.Two days after acetic acid enema,the rats in group 1 began to have the following symptoms of wilting,slow response,reduced activity,bunching,hair color,brittleness,increased stool frequency,thin stools,mucus bloody stool or pus and blood loss and weight loss.After 1-3 weeks,the rats developed colonic ulcer,inflammation,thickening,shortened length,increased colon weight,colonic mucosa congestion,edema,high erosion,single or multiple ulcers.The colon had severe inflammation,and the submucosal,muscular,serosal,and basal 2/3 crypts were destroyed,or only the intact surface epithelium remained.The serum IL-6 and intestinal mucosal TNF-? positive expression scores were significantly increased.All indicators were significantly different from the control group.2.After 1-3 weeks of modeling,the rats in the group 2 had good mental state,normal activities,no obvious bunching,slight matte color,slightly soft stool texture,no stool thinning and mucus,fecal occult blood test(+),and no significant weight loss.There was no difference in colon weight and length compared with the control group.The colonic mucosa was mildly hyperemic,edematous,mildly erosive,and free of ulcer formation.The colonic mucosa was chronically inflammatory,the lesion was confined to the submucosa,and the 1/3 of the basal crypt was destroyed.The concentration of IL-6 in the serum was slightly elevated.The integral value of TNF-? positive expression in intestinal mucosa increased.The above observations were not different from the control group.The above observations were not different from the control group.3.1w after the modeling was completed,,the rats in group 3 showed signs of apathy,unresponsiveness,decreased activity,bunching,lack of color and brittleness,increased stool frequency,thin stools,mucus bloody stool or pus and blood loss and weight loss.And the rats developed colonic ulcer,inflammation,thickening,shortened length,increased colon weight,colonic mucosa congestion,edema,high erosion,single or multiple ulcers.The colon had severe inflammation,and the submucosal,muscular,serosal,and basal 2/3 crypts were destroyed,or only the intact surface epithelium remained.The serum IL-6 and intestinal mucosal TNF-? positive expression scores were significantly increased.All indicators were significantly different from the control group.However,at week 2-3,the body weight of the rats gradually increased,the stool became hard,the mucus pus and blood gradually disappeared,and the occult blood test was positive(+).The length of the colon growed and the weight was reduced.Colonic mucosal congestion and edema were relieved,and the ulcer gradually healed.The lesion was confined to the submucosa and the 1/3 crypt of the basal was destroyed.The serum IL-6 and intestinal mucosal TNF-? positive expression scored gradually decreased.There was no significant difference between the groups at 3w and the control group.4.In each group of Gexiantang treatment group,the colon inflammation,congestion,edema,the lesion range was reduced,the crypt destruction was reduced,the colonic mucosal erosion and ulcer healing,the stool gradually formed,the pus and blood decreased,and the body weight increased.The disease activity index of the GXT high,middle and low dose group was significantly different from that of the control group,P<0.01.There was no difference between the high,middle and low dose groups,P>0.05.5.The number of white blood cells and platelets in the GXT treatment group decreased,and there were significant differences between the high,middle and low dose groups and the control group,P<0.01.There was no difference in the reduction of white blood cells in each treatment group.There was a difference between the reduction of platelets in the high-dose group and the middle-dose and low-dose groups,P<0.05.High and low doses of GXT did not significantly increase the number of red blood cells,and there was no difference between the two groups.The increase in the number of red blood cells in the middle dose group was different from that in the control group and was significantly different from the normal group.6.Serum IL-6 levels were decreased and IL-10 levels were increased in GXT treatment groups.There were significant differences between the high,medium and low dose treatment groups and the control group,P<0.01.The effect of increasing IL-10 levels in the high-dose group was different from that in the middle and low-dose groups,P<0.05.The effect of lowering IL-6 content in the middle dose group was different from that in the high and low dose groups,P<0.05.7.The positive expressions of i NOS and TNF-? in the colon tissue of rats in GXT treatment group were decreased.There were significant differences between the high,middle and low dose groups and the control group,P<0.01.There was a difference between the high and middle dose groups and the low dose group,P<0.05.There was no difference between the high and middle dose groups.8.There were no significant differences in serum SCr,BUN,AST and ALT between the GXT high,medium and low dose groups and the normal group and the control group,P>0.05.Conclusion 1.The disease change of the ulcerative colitis model prepared by inactivated E.coli strain immunization + acetic acid(AA)enema immunocomplex method is similar to human UC,and the duration of the lesion is long.This method is an ideal modeling method.2.Gexian Decoction has the effects of reducing the number of inflammatory cells and platelets,inhibiting the release of inflammatory factors,anticoagulation,and promoting the production of anti-inflammatory factors in rats with ulcerative colitis.3.Gexian Decoction can promote the repair of mucosal ulcerative colitis.4.Gexian Decoction has no obvious damage to liver and kidney function.In conclusion,the experimental results show that Gexian Decoction can affect inflammatory cells,inflammatory factors,platelets and colonic pathological changes,and has a certain therapeutic effect on ulcerative colitis. |
PDF Full Text Request