The Effects Of Fomes Officinals Ames Polysaccharides On The Inflammatory Response In BV-2 Microglial Cells Induced By A?_25-35

Objective:To investigate the inhibitory effects of Fomes Officinals Ames polysaccharides?FOPS?and Fomes Officinals Ames polysaccharides components?FOP-a?on the inflammatory response in BV-2 microglial cells induced by A?_25-35 in condensed state as inflammatory cell model in vitro.Methods:BV-2 microglia cells were treated with different concentrations of FOPS and FOPS-a?6.25?12.5?25?50?100?200?400?g/mL?and cell viability was tested by MTT.A harmless concentration?6.25?12.5?25?mol/L?of FOPS and FOPS-a were used for intervention on the follow-up inflammatory model.The changes of BV-2 microglia cellular morphology were observed under inverted phase contrast microscope.The levels of IL-1?IL-6?TNF-?and MCP-1 expression were measured by ELISA assay.The lactate dehydrogenase activity in BV-2 cells were measured by LDH kit assay.The expression of inflammatory IL-6?TNF-a?MCP-1?iNOS?COX-2 and NF-?B were measured by QPCR.The expression of the iNOS?COX-2?I?B?and NF-?B were analyzed by Western blot assay.Results:MTT assay demonstrated that FOPS and FOP-a had no toxic effect on cell viability exposure to the concentration was6.25²5?g/mL.Compared with model group,the?6.25?12.5?25?g/mL?FOPS and FOP-a could inhibit the morphological changes of BV-2 cell induced by A?_25-35.The gene expression of IL-6?TNF-a?MCP-1?iNOS?COX-2 and NF-?B in BV-2 cells and the production of pro-inflammatory cytokines IL-1?IL-6?TNF-??MCP-1 and LDH activity expression in A?_25-35 stimulation groups were significantly attenuated by FOPS and FOPS-a?p<0.05?.The increasing expression of iNOS and COX-2 and nuclear translocation of NF-?B p65 were suppressed by FOPS and FOP-a as well as the decreased expression of I?B?incytoplasm was attenuated by FOPS and FOP-a?p<0.05?.Conclusion:1.A?_25-35 could activate BV-2 cell and up-regulate the expression levels of inflammation factors such as IL-1,IL-6,TNF-?and MCP-1.2.FOPS and FOP-a could inhibit the expression of IL-1,IL-6,TNF-?and MCP-1 induced by A?_25-35 activated BV-2cell.2.FOPS and FOP-a could inhibit the expression of IL-1,IL-6,TNF-?,MCP-1induced by A?_25-35 activated BV-2 cell.3.FOPS and FOP-a could inhibit the inflammatory response of BV-2 cell induced by A?_25-35 by inhibiting the expression of NF-?Bp65/I?B?in NF-?B signaling pathway.4.FOPS and FOP-a inhibit the expression of COX-2 and iNOS by inhibit NF-?B signaling pathway and further inhibit the inflammatory response of microglial induced by A?_25-35.