Construction And Spectral Analysis Of Antibacterial Activity Molecular Detection System

As an important antibacterial or bacteriostatic active drug,antibacterial drugs play an important role in human disease prevention,treatment and maintenance of human health.With the continuous improvement of medical level,the potential side effects of various antibacterial agents are more clear.The sensitive and rapid detection of antimicrobial agents is a basic premise for the calibration of drugs in the process of drug taking,injection,dose control and pharmaceutical preparations.In recent years,with the development of detection technology in the field of drug analysis,various detection methods have been constantly presented,but rapid and simple detection methods are still the research direction of many scholars.Based on the superior phosphorescence properties of Mn doped Zn S quantum dots,a sensitive detection method of Lomefloxacin,norfloxacin and tobramycin hydrochloride was constructed and its mechanism was discussed.The concrete results are as follows:(1)In view of the charge transfer function of several benzene rings,pyridine rings and carbonyl groups in the molecular structure of Lomefloxacin,a simple and rapid phosphorescence detection method for the determination of lomefloxacin hydrochloride was established,and the detection conditions were optimized.Under the condition of p H 7.4,the reaction was 10 min,and the concentration of lomefloxacin hydrochloride was in a good linear relationship with the ratio of phosphorescence quenching intensity of Mn doped Zn S quantum dots in the range of0.002~1.4μg/m L,and the detection limit was 0.7×10-3 μg/m L.The method can avoid the interference of some foreign substances,and can realize the rapid determination of lomefloxacin hydrochloride eye drops and lomefloxacin hydrochloride in human urine.(2)Based on the charge transfer between Mn doped Zn S quantum dots andnorfloxacin nicotinate,a method to detect norfloxacin nicotinate was established.In the detection system,norfloxacin nicotinate,as an electron acceptor,can accept the excited state electrons of Mn doped Zn S quantum dots,and quench the phosphorescence of quantum dots.The detection range of norfloxacin nicotinate is0.1~4 μg/m L,and the detection limit is 1.8×10-2μg/m L.This method can be used for fast calibration of norfloxacin injection and niacin norfloxacin nicotinate.(3)Based on the electrostatic surface effect between Mn doped Zn S quantum dots and tobramycin,a fast and simple method for the determination of tobramycin was established.In the construction of the method,tobramycin,as a small molecule with positive electricity,can interact with the Mn doped Zn S quantum dots,which are negative on the surface,and induce the quantum dots to accumulate and enhance the luminescence of the quantum dots.Moreover,the phosphorescence intensity of QDs showed a good linear relationship with the concentration of tobramycin at 0.5~8μg/m L,and the detection limit was 0.004μg/m L.The detection method of the design can be applied to tobramycin injection and tobramycin in human urine.