| Background & ObjectiveHepatocellular carcinoma(HCC)is the most common pathological type of hepatocellular carcinoma,and its molecular mechanism remains to be elucidated.Because of its molecular mechanism is unclear,the prognosis of HCC patients is still poor.Therefore,the cancer related mortality was second in men with HCC.At present,the treatment of HCC is limited,because most patients are diagnosed at the late stage,the treatment of HCC is greatly limited.At present,the main treatment is still limited to surgical resection,TACE,radiation therapy,liver transplantation,etc.In addition,HCC is easy to metastasis and recurrence,and it is also one of the factors that lead to a high degree of malignancy and poor prognosis.There is an urgent need to explore a key gene that affects the occurrence and development of HCC,and as a molecular marker for HCC diagnosis and prognosis,to monitore expression of the gene to increase the diagnosis rate of patients and the prognosis.The potential of transforming growth factor binding protein 2(LTBP2)is a member of the latent transforming growth factor binding protein family,which belongs to extracellular matrix(ECM),LTBP2 can regulate the biological activity of TGF-beta function,and also affect the structural integrity of the ECM.TGF-beta plays an important role in the development of HCC,and LTBP2 can indirectly regulate the activation of TGF-beta.In addition,LTBP2 contains RGD integrin recognition sites,which plays an important role in cell adhesion.The function of LTBP2 has been found in many tumors,such as nasopharyngeal carcinoma(NPC)and esophageal squamous cell carcinoma(SCC).At present,the expression of LTBP2 in hepatocellular carcinoma(HCC)has been found to be elevated in the serum and tissues.However,the relationship beetween prognosis of patients with HCC with LTBP2 and the function and mechanisms are still unclear.Therefore,our study aims at the analysis of clinical samples,combined with clinical data to predict the prognosis of the HCC patients,and to reveal its function and mechanism in HCC,so as to find new key targeting gene for HCC.Methods1.The expression levels of LTBP2 at the mRNA and protein levels were detected by RT-PCR,Western blot and immunohistochemical staining.2.The correlation between LTBP2 expression and clinical data of patients with hepatocellular carcinoma was analyzed by chi square test.3.Using the Kaplan-Meier curve analysis of the relationship between the expression of LTBP2 and overall survival(OS)in patients with HCC.4.Regulate the expression of LTBP2 in hepatocellular carcinoma cell lines,and the proliferation ability of the cells was detected by MTT and crystal violet staining.5.Analyze the changes of the signal transduction pathway by Western Blot.Results1.LTBP2 was increased in HCC tissues,and there was a significant correlation between the expression of LTBP2 and the diameter of HCC;2.The overall survival(OS)of patients with high expression was shorter than that of patients with low expression of LTBP2,and LTBP2 could be used as an independent prognostic factor for HCC patients;3.Regulating the expression of LTBP2 in hepatocellular carcinoma cell lines can affect the proliferation of HCC cells;4.LTBP2 can promote the proliferation of HCC cells by up-regulating the expression of pEKR1/2 and pAKT.ConclusionsThe expression of LTBP2 in HCC tissues was significantly higher than that in adjacent non-tumor tissues.The expression level of LTBP2 could predict the overall survival time of patients with HCC,and LTBP2 could promote the proliferation of HCC cells by promoting phosphorylation of extracellular regulated protein kinases(pERK1/2)and protein kinase B(pAKT).LTBP2 may be a new target for the treatment of HCC. |
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