Effect Of VEGFA Monoclonal Antibody On Vascular Endothelial Cells Regulating The Invasion And Migration Of Glioma Cells

GBM(Glioblastoma)is a very common and aggressive tumor with a hi gh degree of malignancy.Glioma can lead to increased intracranial pressure,cerebral hemorrhage,brain edema,and neurocognitive deficits,which limit the use of many ca ncer therapies to some extent.Anti-angiogenesis therapy is a main method for the treatment of glioma at present,which can inhibit the tumor relative angiogenesis to inhibit the tumor growth,and Bevacizumab is wildly used drug for tumor anti-angiogenesis therapy,the nature of Bevacizumab is a humanized VEGF monoclonal antibody,which can combine with VEGF to prevent VEGF from binding to the corresponding receptor.In the treatment of glioma with Bevacizumab has achieved a certain effect,while some of the problems of tumor recurrence and metastasis will still appear,as for the specific mechanism involved is still not clear.The theory that the tumor cells are not only influenced by external stimuli and its gene mutation leads to tumor formation and metastasis,tumor microenvironment also has a very important impact,vascular endothelial cells can form new blood vessels in the tumor and then provide nutrition for tumor cells to promote tumor genesis,and tumor cells can transfer to other organ through the new blood vessels.Whether there exist some cross-talk between the endothelial cells and tumor cells and how these cross-talk function in promoting tumor genesis,the mechanism is not very clear.In order to study the effect of cross-talk between endothelial cells and glioma cell in migration and invasion of glioma cell,and how Bevacizumab treatment influence this process,in this study,we used VEGFA monoclonal antibody to simulating the Bevacizumab to study the effect of Bevacizumab in co-culture of HUVEC and U87 in promoting U87 migration and invasion.The results show that HUVEC co-cultured with U87 cells can promote the invasion and migration of U87 cells,while adding VEGFA monoclonal antibody can partially inhibit this promote process.It is also found that in the co-culture system,Wnt signaling pathway in U87 is activated,while the Notch signal did not change significantly,indicates that Wnt signaling pathway may be involved in the crosstalk between endothelial cells and tumor cells.This is the first study of the effect of Bevacizumab on glioma cells in simulated in vivo environment and the possible mechanisms involved,Wnt signaling pathway ma y play a role in the treatment of glioma in the Bevacizumab,this study provided a new thinking of bevacizumab in treating glioma,and can provide some early theoretical basis for the combination treatment of glioma.