Study On Chloridechannel Blocker NPPB Inhibition Of Postoperative Peritoneal Adhesion

Objective: To study the inhibitory effect of chloride channel blocker NPPB on postoperative peritoneal adhesion,explore its possible mechanism,and provide evidence for NPPB as a new drug for postoperative peritoneal adhesion prevention.Methods: 1)Rat peritoneal adhesion model was established and treated with different concentrations of NPPB to observe the effect of NBBP on peritoneal adhesion formation.2)Primary culture of rat peritoneal adhesion fibroblasts was performed with tissue mass cell culture to obtain peritoneal adhesion fibroblasts.3)Identification of peritoneal adhesion fibroblasts by immunofluorescence.4)MTT assay was used to observe the effect of NPPB on proliferation of peritoneal adhesion fibroblasts.5)The effect of NPPB on the migration of peritoneal adhesion fibroblasts was observed by cell scratch assay.6)Whole-cell patch clamp technique was used to analyze the effect of NPPB on volume-activated chloride current in peritoneal adhesion fibroblasts.7)Real-time cell volume monitoring technique to observe the effect of NPPB on regulatory volume decrease(RVD)of peritoneal adhesion fibroblasts.Results: 1)Rat peritoneal adhesion model was successfully established by using knife scraping method.2)NPPB significantly inhibited the formation of postoperative peritoneal adhesions.3)Positive expression of vimentin in peritoneal adhesion fibroblasts.4)NPPB significantly inhibited the proliferation of peritoneal adhesion fibroblasts.5)NPPB significantly inhibited migration of peritoneal adhesion fibroblasts.6)NPPB inhibits volume-activated chloride current in rat peritoneal adhesion fibroblasts.7)NPPB inhibits RVD in rat peritoneal adhesion fibroblasts.Conclusion: The chloride channel blocker NPPB can effectively inhibit the formation of postoperative peritoneal adhesions by inhibiting the volume-activated chloride current of peritoneal adhesion fibroblasts.Resulting in a decrease in cell RVD,this in turn reduces the proliferation and migration of the cell,and ultimately inhibits the formation of peritoneal adhesion.It is suggested that NPPB has the potential to be developed for the prevention and treatment of clinical peritoneal adhesion.