The Effect Of CD36 Over-expression On Granulosa Cells In Obese Patients Without PCOS And Its Mechanism

Part?: Gene expression of granulose cells differs in obese and normal weight patients without PCOSObjective: To screen the different expressed genes(DEGs)of granulose cells(GCs)between obese and normal weight patients without PCOS.Methods: Affymetrix Gene Chip is used to detect the gene expression profile of GCs from human ovarian follicular fluid that was collected after centrifugation through 50% Percoll gradient.Using bioinformatics to analysis the outcomes of gene expression profile.We use real-time PCR technique to validate the credibility of Gene Chip outcomes.Results: There are 16 DEGs in the two groups,of which the fold change of BUB1,SFRP4,CD36 and PLA2G7 is above two,respectively.We find five pathways,including BUB1 and CD36 after bioinformatics analysis.Three of the five pathways are associated with oocyte maturation,one of the five pathways is associated with fat digestion and absorption.The results of q RT-PCR are consensus with Gene Chip about the expression of BUB1,CD36 and CDK1.Conclusion: Oocyte maturation related pathways and fat digestion and absorption pathway change significantly in granulosa cells of obese patients without PCOS compared with normal weight patients.BUB1 and CD36 may play a important role in it.Part? The effect of CD36 over-expression and its inhibitor SSO on COV434 and its mechanismsObjective: To study the effect of CD36 over-expression and SSO on the amount of triglyceride(TG),cell proliferation and apoptosis and endocrine function of COV434.Methods: The CD36 plasma transfects COV434 that is incubated in medium including palmitic acid.Furthermore,to detect the amount of TG in COV434,cell proliferation,cell apoptosis,the amount of estrogen(E2)in medium.When SSO was used to prohibit CD36 functions,the above markers were detected by the same methods,respectively.Results: The expression of CD36 protein in experimental group is 2.79 times as compared with control group after 24 hours,which leads to higher TG level in experimental group than control group.The cell viability declines 16.2% and cell apoptosis rate increases 7.21% in experimental group.Moreover,the amount of estrogen is lower in experimental group.Surprisingly,SSO can inhibit the function of CD36.Conclusion: CD36 over-expression increases TG in COV434,inhibits cell proliferation,promotes cell apoptosis,and inhibits the secretion of estrogen.Surprisingly,SSO can inhibit the function of CD36 over-expression on COV434.We suggest that CD36 may be a important factor in the aspect of regulating the fecundity of obese patients without PCOS.