Significance And Expression Of Caveolae Regulated Protein:Serum Deprivation Response Protein (SDPR) In Hepatocellular Carcinoma

AimTo study the expression of caveolae regulated protein: serum deprivation response protein(SDPR)in hepatocellular carcinoma(HCC)tissues.To study the expression of two other caveolae related proteins: polymerase?and transcript release Factor(PTRF)and caveolin-1(Cav-1)in HCC tissues.To study the correlation among expression of SDPR,PTRF and Cav-1 in HCC tissues,as well as their correlation with clinical and pathological characteristics of HCC patients,and explore the possible effects of caveolae dysfunction on the HCC development.Methods1.Using RNA sequencing(RNA-Seq),differentially expressed genes(DEGs)between HCC tissues and adjacent non-tumor tissues of 9 HCC patients were screened,and then SDPR was identified as a significantly down-regulated DEGs existed in 9 HCC tissues,which has been achieved in our previous study.2.SDPR m RNA expression in tumor tissues and adjacent non-tumor tissues of 80 HCC patients was validated by Real-time Quantitative Polymerase Chain Reaction(RT-q PCR).Meanwhile,the expressions of PTRF m RNA and Cav-1 m RNA were detected by same method.3.SDPR protein expression in tumor tissues and adjacent non-tumor tissues of 80 HCC patients was detected by Immunohistochemistry(IHC).Results1.By RNA-Seq,we identified 1943 significantly DEGs between HCC tissues and adjacent non-tumor tissues,of which 690 genes were up-regulated,and 1253 genes were down-regulated.DEGs were rich in various metabolic processes,cell cycle and signaling pathways.Especially,cell cycle and P53 signal pathway closely related to cancer.SDPR expression levels in HCC tissues and adjacent non-tumor tissues were 3.39(1.57-6.61)and 12.35(10.02-14.43)respectively,which existed a significance difference(P<0.05).2.SDPR m RNA expression in HCC tissues was validated by RT-q PCR.There were 56 cases with low SDPR m RNA expression and 24 cases with high SDPR m RNA expression in 80 HCC tissues,which was significantly lower than that in adjacent non-tumor tissues(P<0.05).3.SDPR protein expression in HCC tissues was detected by IHC.There were 66 cases with low SDPR protein expression and 14 cases high SDPR protein expression in 80 HCC tissues,which was significantly lower than that in adjacent non-tumor tissues(P<0.05).4.The expressions of PTRF m RNA and Cav-1 m RNA in HCC tissues were detected by RT-q PCR.For PTRF m RNA,among 80 cases of HCC tissues,there were 53 cases with low expression and 27 cases with high expression,which was significantly lower than that in adjacent non-tumor tissues(P<0.05).For Cav-1 m RNA,among 80 cases of HCC tissues,there were 35 cases with low expression and 45 cases with high expression,which was no significantly different from that in adjacent non-tumor tissues(P>0.05).5.It existed significant positive correlation among expression of SDPR,PTRF and Cav-1 in HCC tissues(P<0.05).There was a significant positive correlation among SDPR m RNA,PTRF,and serum cholinesterase(CHE)activity in HCC patients(P<0.05).Conclusions1.Comparing with adjacent non-tumor tissues,the expressions of SDPR m RNA and protein were significantly down-regulated in HCC tissues.2.Comparing with adjacent non-tumor tissues,the expressions of PTRF m RNA was significantly down-regulated in HCC tissues.3.There was no significant difference of Cav-1 m RNA expression between HCC tissues and adjacent non-tumor tissues.4.It existed a significant correlation among SDPR,PTRF and Cav-1 expression in HCC tissues,which might play important role on the HCC process by influencing the caveolae structure.5.CHE activity was significantly reduced and positively correlated with SDPR m RNA and PTRF m RNA in HCC patients,which suggest that caveolae dysfunction and cholinergic system were involved in the development and progression of HCC.