Bone Marrow Mesenchymal Stem Cells Promote The Invasion Of Prostate Cancer Cells Through CXCL12

Objective: Since twentieth Century,the incidence of prostate cancer has increased significantly in China.And the survival time of the prostate cancer patients is long,in the long-term course of disease,it is easy to distant metastasis,bone is a common place for metastasis of prostate cancer.There is no effective method to prevent bone metastases.On the basis of previous studies,this study aims to confirm that bone marrow mesenchymal stem cells can promote the invasion of prostate cancer cells,and further explore specific mechanisms to find a scheme for the prevention and treatment of prostate cancer bone metastases.Content: 1.To confirm that bone marrow mesenchymal stem cells can promote the invasion of prostate cancer cells.2.To explore the mechanism of bone marrow mesenchymal stem cells promoting the invasion of prostate cancer cells.3.Screening drugs that can inhibit the invasion of prostate cancer cells,and provide a feasible solution to solve the clinical problems of prostate cancer bone metastasis.Method: 1.The effects of different conditioned medium on C4-2 cells' invasiveness were compared by Transwell.And the transcription and expression changes of E-cadherin and N-cadherin were detected by PCR and Western blot.2.The effects of different conditioned medium on C4-2 stem cells were compared by sphere formation assay.And the transcription of CD44 were detected by QPCR.3.Cytokine related to invasion in different conditioned medium were analyzed by cytokine array,and confirm cytokine promotes prostate cancer cell invasion by cell experiment.Result: 1.The BMSC-CM can promote the invasion of C4-2 cells and reduce the transcription and expression of E-cadherin and increase the transcription and expression of N-cadherin.2.The BMSC-CM can maintain the stemness of C4-2 stem cells and maintain the expression of CD44.3.The expression of CXCL12 in BMSC-CM increased significantly,and CXCL12 inhibitor could inhibit C4-2 invasion.Conclusion: 1.Bone marrow mesenchymal stem cells can promote the invasion of prostate cancer C4-2 cells.2.Bone marrow mesenchymal stem cells promote the invasion of prostate cancer C4-2 cells mainly through CXCL12.