| Objective To detect the expression of cir-ITCH in the tumor tissues of gastric cancer patients and analyse the correlation between cir-ITCH expression and the clinicopathological features.To explore the potential clinical value of cir-ITCH in gastric cancer and offer a new biomarker for the diagnosis of gastric cancer.We also detected the expression of cir-ITCH in huc MSC and GC-MSC,and investigated the roles in the malignant transformation in human umbilical cord mesenchymal stem cells,in order to provide a new basis for the clinical application of mesenchymal stem cells.Methods QRT-PCR was used to detect the expression level of cir-ITCH in gastric cancer tissues.The correlation between cir-ITCH expression levels and the clinicopathological factors was analyzed.Gastric cancer cells SGC-7901,MGC-803 and human umbilical cord mesenchymal stem cells were transfected with si RNA that directed against cir-ITCH,and MGC-803 were transfected with overexpressed cir-ITCH.The interference and overexpressed efficiency were verified by q RT-PCR.The growth curve and cell colony formation asaay were used to detect the cell proliferation.Transwell migration/invasion assays were used to measure the ability of cell migration and invasion after transfection.We preliminary predicted mi RNAs which may interacted with cir-ITCH through Database and literature reading.QRT-PCR and Western Blot were used to detect the genes and proteins in downstream signaling pathways.Results The expression levels of cir-ITCH was downregulated in gastric cancer tissues compared to paired non-tumor tissues(P<0.05).cir-ITCH expression in gastric cancer tissues was closely related to TNM stage(P<0.05),while not related to the other factors(P>0.05).The expression of cir-ITCH was downregulated after cir-ITCH knockdown,while that was upregulated after cir-ITCH overexpressed.And the results showed that cir-ITCH could inhibit gastric cancer cells proliferation,migration and invasion ability by inhibiting the activation of TGF-?/Smad signaling pathways.Further study found that cir-ITCH may act as a molecular sponge for mi R-7/mi R-214,and mi R-7/mi R-214 inhibitors could reverse gastric cancer cells proliferation,migration and invasion ability which promoted by si-cir-ITCH.Also,cir-ITCH showed a downregulated trend in the GC-MSC compared with huc MSC.The knockdown of cir-ITCH also promoted huc MSC proliferation,migration and invasion by activating TGF-?/Smad signaling pathways.Conclusion cir-ITCH was downregulated in gastric cancer tissues and was closely related to TNM stage(P<0.05).We found that cir-ITCH may act as a molecular sponge for mi R-7/mi R-214 to inhibit gastric cancer cells proliferation,migration and invasion ability through inhibiting the activation of TGF-?/Smad signaling pathways.cir-ITCH was also downregulated in GC-MSC compared with huc MSC and knockdown of cir-ITCH promoted huc MSC proliferation,migration and invasion by activating TGF-?/Smad signaling pathways. |
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