Expression And Clicnical Significance Of TIM-3 And PD-1 In Breast Invasive Ductal Carcinoma Patients

Objective:To detect the expression of T cell immunoglobulin and mucin domain-3(TIM-3)and Programmed Death-1(PD-1)on the T cell of T lymphocytes of peripheral blood and tumor tissues from breast invasive ductal carcinoma patients,analyze the relationship between the expression and the clinicopathological characteristics of the tumor,investigate the role of TIM-3 and PD-1 in the progression of invasive ductal carcinoma of the breast.Methods:1.All peripheral blood of 40 breast cancer patients and 20 healthy people was obtained.T lymphocytes were obtained by the gradient protocol.The expression of TIM-3 and PD-1 on T lymphocytes were analyzed by flow cytometry,and the relationship between them and clinicopathological features were analyzed.2.Tumor tissue and adjacent normal tissue of 10 breast invasive ductal carcinoma patients was obtained.The single suspensions were obtained when these tissue were mechanical grind and digested with collagenase digestion solution.Tumor infiltrating lymphocytes(TILs)were further purified by the gradient protocol.The expression of TIM-3and PD-1 on T lymphocytes were analyzed by flow cytometry,and the relationship between them and clinicopathological features were analyzed,the effect of the tumor on the microenvironment of the tumor were investigated.Results:1.The frequencies of TIM-3+ cells among T lymphocytes in breast cancer patients peripheral blood were significantly higher than healthy people,and the difference was statistically significant(2.01±0.62% vs0.26±0.08%,P=0.03).2.The frequencies of TIM-3+ cells of T lymphocytes in low differentiated breast cancer patients peripheral blood were also higher than that in middle –high differentiated patients,the difference was statistically significant(4.45±1.22% vs1.02±0.27%,P=0.005).3.The number of PD-1+ and TIM-3+ T cells showed a significant positive correlation(r=0.47,P=0.02).4.The expression of TIM-3 in breast cancer tissues and normal peritumoral tissue T cells was 30.79±8.48% and 6.21±3.83%,respectively,and the difference was statistically significant(P=0.03).5.The expression of TIM-3+PD-1+ in breast cancer tissues and normal peritumoral tissue T cells was 44.89±12.42% and 3.26±2.25%,respectively,and the difference was statistically significant(P=0.03).Conclusion:1.The expression of TIM-3 in the peripheral blood T cells of thepatients with breast invasive ductal carcinoma was significantly higher than that of the healthy people,and the patients with low differentiation tumor were higher than those with middle and high differentiation,suggesting that it has a significant correlation with the development of the tumor,and it promotes the progression of breast invasive ductal carcinoma of breast.2.The expression of TIM-3 and TIM-3+PD-1+ in the tumor tissues of breast invasive ductal carcinoma is significantly higher than that of the normal tissue around tumor tissue,which makes the immunosuppression in the tumor microenvironment stronger than the normal tissue around the tumor,which plays a significant role in the development of the tumor.