Effects Of Ea On The Circadian Rhythm Of The HCC Mice And The Epigenetic Modification Of The SCN

Objective:To hepatocellular carcinoma in situ?Hepatocellular carcinoma HCC?mouse model to observe the effect of electroacupuncture adjusting effect on liver cancer in mice with spontaneous wheel activity rhythm and visual suprachiasmatic nucleus?Suprachiasmatic-nucleus,SCN?in the effect of apparent genetic related gene expression,discuss the electroacupuncture regulation of mice spontaneous wheel activity rhythm of epigenetic mechanisms.Methods:After the 108 male C57BL/6J mice were strictly selected according to6 zeitgeber time?Zeitbeger Time,ZT?were randomly divided into ZT0 group?07:00?,ZT4?11:00?group,ZT8 group,ZT12?15:00??19:00?group,ZT16?23:00?group and ZT20?03:00?group.Each group was respectively set blank control group,model group and EA group.Male C57BL/6J mice were placed in the rhythm of screening qualified laboratory mice within the isolation unit,the lighting environment settings for the alternating light and dark state?Light and Dark LD 12:12,7:00¹9:00 as 19:00^,7:00the next day dark period?began 10 days after domestication;domestication began modeling,the 6 group model group zeitgeber with the electroacupuncture group mice were injected into the leaves along the H₂₂ axis of 10?L mouse liver cancer cell suspension copy orthotopic transplantation mice model of hepatocellular carcinoma,the control group were injected with 0.9%normal saline 10?L.After the success of the model,the electroacupuncture group were given Electroacupuncture of mice"ganShu"and"Zhiyang"at corresponding time points,the blank group and the model group tied to the same time,under the same conditions.The mice were at the corresponding time point of tenth 2h after EA were sacrificed,derived from the measured.The ClockLab?ACT-500?software for continuous recording of mouse spontaneous activity rhythm,electro-acupuncture after 10 days by MATLAB?R2007b?derived from mouse wheel activity rhythm of spontaneous activity rhythm analysis runner orthotopic transplantation mice starting time,amplitude,phase,peak period.According to the best time of electro acupuncture intervention group choose rhythm parameters using a high-throughput,epigenetic gene expression related to epigenetic PCRarray array detection of mouse SCN parts of the table.Result:?Before modeling,22 mice each runner activity rhythm starting time,period,amplitude,peak phase values,there were no significant differences?P>0.05?,mouse wheel activity shows circadian rhythm characteristics.?After modeling,orthotopic transplantation of liver cancer mouse wheel activity rhythm of each model group showed decreased peak amplitude and phase lag;the model group respectively before modeling and amplitude difference before and after comparison,the difference compared with the control group amplitude,the differences were statistically significant?P<0.05?;the animal model group the peak value and phase respectively before and after modeling,compared with the control group the difference of peak phase difference compared with the control group,the difference of amplitude,the differences were statistically significant?P<0.05?;but the animal model group starting time,and cycle respectively before modeling,compared with the control group the difference between before and after the animal activity starting time,cycle the value of difference comparison,the differences were not statistically significant?P>0.05?.?After acupuncture,orthotopic transplantation of mice in the EA groups the activity rhythm of the runner is reduced and the peak amplitude increased lag phase forward;the amplitude value of mice in the EA group was respectively with electroacupuncture before the difference between before and after comparison,difference compared with model group amplitude,the differences were statistically significant?P<0.05?;ZT0,ZT4 ZT12,ZT20,ZT16,electro acupuncture group and electroacupuncture mice respectively before the peak phase difference before and after comparison,the difference compared with the model group the peak value of the phase,there were no significant differences?P>0.05?;and ZT8 EA group mice respectively before EA and peak phase difference before and after comparison,the difference compared with the model group with peak phase values.Statistically significant difference?P<0.05?;and in six different time points,to adjust the ZT8 EA orthotopic transplantation mice wheel activity rhythm is the most effective performance for the ZT8 EA group can significantly increase the amplitude of mice decreased runner activity rhythm,the peak phase advance lag.The electroacupuncture group activities in mice starting time,and cycle respectively before and after acupuncture,difference with the model group MICE activities starting time,cycle difference compared with the control group,MICE activities starting time,cycle difference comparison,the differences were not statistically significant?P>0.05?.?After modeling,the mice in ZT8 model group SCN 84 epigenetic related genes in which 33 upregulated and 6 genes related to DNA methylation,histone acetylation related gene 13;histone methylation related gene 14.?After acupuncture,electroacupuncture group ZT8 mice SCN 84 epigenetic gene31 expression,including 5 genes related to DNA methylation,histone acetylation related gene 11;histone methylation related gene 15.Conclusion:?The normal mice spontaneous activities showed the runner circadian rhythm characteristics.?The successful establishment of orthotopic liver cancer mouse model,the spontaneous activity rhythm of runner showing reduced amplitude,peak phase lag phase characteristics.?The spontaneous activity rhythm after acupuncture,the runner performance orthotopic transplantation mice:the peak phase amplitude increased,reduce the forward backward,the adjustment function in ZT8 time point electroacupuncture on orthotopic transplantation of mice spontaneous wheel activity rhythm of the most significant performance for the amplitude increased most significantly,only ZT8 time point can peak phase forward backward.?The effect of Electroacupuncture on orthotopic transplantation of mice with SCN liver cancer in view of genetic genes related to certain tables,such as DNA methyltransferase 3B?Dnmt3b?,histone deacetylase 3?Hdac3?,histonelysine methyltran ferase 1a?Kdm1a?,histonely sinede methylase 2e?Kmt2e?gene downregulation,may participate in the adjustment of EA orthotopic transplantation mice spontaneous wheel activity rhythm within the SCN epigenetic molecular mechanism.