Umbilical Cord Mesenchymal Stem Cells(UC-MSCs) Improve HepG2 Cells And Type 2diabetes Mice Insulin Resistance By Increasing Autophagy

In varity of studies,umbilical cord mesenchymal stem cells(UC-MSCs)was be found can improve insulin resistance in type 2 diabetic mice.This effect may be achieved by promoting the autophagy of insulin target cells to improve the homeostasis of the cells.Autophagy is the process by which cells metabolize their own cytoplasm and organelles by lysosomes.Moreover,autophagy is closely related to insulin resistance(IR).This study was conducted to treat type 2 diabetic mice by using umbilical cord mesenchymal stem cells.The experimental results showed that insulin resistance(IR)was improved after treatment.HE staining and PAS staining showed improvement of liver structure and inflammation.The western-blot results showed that the autophagy associated protein LC3 B,ATG7 and bcl-1 were significantly increase in mouse liver,and the autophagosome was increased in electron microscopy.At the same time,UC-MSCs therapy the HepG2 cells with insulin resistance induced by palmitic acid(PA),and the insulin resistance of HepG2 cells was relieved and the autophagy was increased.After chloroquine(CQ),thebenefits of UC-MSCs also disappeared.This study suggests that UC-MSCs can improve insulin resistance in type 2 diabetes by enhancing autophagy.