The MDA-435-spheroid enrichment(MDA-435-SE)cells were isolated from human breast cancer cells MDA-MB-435 through continuous spheroid-enrichment cell culture.Then the MDA-435-SE-shSec23a cell line in which the Sec23a was knocked down and the negative control cell line MDA-435-SE-LV3NC were establishes through lentivirus infection.Cell Counting Kit-8 proliferation assay,96-wells plate single cell cloning assay,and nude mice subcutaneous tumor formation assay were conducted to figure out the effects of Sec23a stable knockdown on the stemness of MDA-435-SE cells.The results showed that Sec23a stable knockdown did not affect the cell proliferation rate of MDA-435-SE cells,but significantly enhanced the single cell cloning ability and tumorigenic ability in vivo of MDA-435-SE cells.The study illustrates that the stable knockdown of Sec23a enhances the stemness of human breast cancer stem-like cells... |