The Study Of Mesoporous Nanomaterials MCM-41 Caarry Two Drugs

OBJECTIVE: To study the drug loading?release properties of MCM-41 mesoporous nanomaterials on paclitaxel(PTX)?cisplatin(CDDP)and the antitumor activity of compound drug(PTX@MCM-41/CDDP@MCM-41).for making the compound nanoscale drug sustained-release preparation.to improve the utilization of drugs and reduce the side effects of drugs.and provide basic experimental basis for MCM-41 carrying anticancer drugs.METHOD: Taking MCM-41 mesoporous nano material as carrier,paclitaxel and cisplatin was used as the target drug,visible ultraviolet spectrophotometer was used as the detection means,carrying capacity and encapsulation rate as the detection index,The effects of time?temperature?loading ratio?ultrasonic on the drug loading performance were investigated;drug release through dialysate bag into simulated body fluid for release in vitro.Testing the inhibitory effects of MCM-41?paclitaxel?cisplatin and compound drugs(MCM-41@PTX)on the proliferation of A549 cells in vitro were detected by MTT and Hoechst 33342.RESULT: 1.The best conditions for PTX are as follows: carrying ratio m(MCM-41): m(PTX)= 1:4,temperature 45 degrees,time 5 h,ultrasonic,at this time the carrying rate is 30.12 %,2.The best conditions for CDDP are as follows: carrying ratio m(MCM-41): m(CDDP)= 1:3,temperature 65 degrees,time 4 h,ultrasonic,at this time the carrying rate is 43.37 %,3.In vitro simulated dissolution experiments show that: the two single drugs have been basically exuded after 24 h,the dissolution rate of paclitaxel in the drug PTX@MCM-41 was only 62.39 % after the simulated oscillating 24 h,the dissolution rate of cisplatin in CDDP@MCM-41 was only 35.26 % at 24 h,compared with single drug,the dissolution rate of compound drugs is low,the effect of sustained release is obvious,and the effect is more lasting.4.MTT cytotoxicity experiments show that: the survival rate of A549 cells wasmore than 86 % under the concentration of MCM-41 at 100 ?g/mL.the activity of A549 cells was respectively only 23.2 % and 23.6% after the compound drug PTX@MCM-41 and CDDP@MCM-41 acted 72 h at 60 ?g/m L.at the same mass concentration and the same time,the compound drug showed stronger antitumor activity than the single drug.5.Hoechst 33342 cell apoptosis experiments show that: the inhibitory rate of compound drug(PTX@MCM-41 and CDDP@-MCM-41)was stronger than that of single drug on the tumor cells under the same mass concentrationand the same time,and the apoptosis was more obvious.CONCLUSION: MCM-41 mesoporous nanomaterials,with low toxicity and good biosafety,are good materials for drug delivery;The effects of MCM-41 carries antineoplastic drugs are affected by various conditions,Compound drugs that were prepared under the best conditions have slow release,long action time and obvious sustained release effect,compound drugs showed higher anticancer activity and stronger antitumor ability through in vitro cell experiments.therefore,MCM-41 carrying anticancer drugs has high potential application in the treatment of tumors.