PRMT1 Coordinates The Epithelial-mesenchymal Transition/Proliferation Dichotomy In Gastric Cancer Cells

Objective:To investigate the protein level of protein arginine methyltransferase 1 in AGS,MGC803,BGC823 and SGC7901 cells.To study the role of protein arginine methylation transferase 1 in the proliferation,migration and invasion of gastric cancer cells,and to analyze the role of protein arginine methylation transferase 1 in the process of epithelial mesenchymal transition(EMT)and look for the possible mechanism for the production of this effect.Methods:Firstly,we analysed the expression levels of PRMT1 in AGS,MGC803,BGC823 and SGC7901 cells by Western blot.The sh-PRMT1 lentivirus was infected AGS and MGC803 cells that express higher level of PRMT1.Stable cells were selected by puromycin.The plasmid Flag-PRMT1 was tranfected into BGC823 and SGC7901 cells,that express lower level of PRMT1.And the PRMT1 expression level was detected by Western blot and qPCR.To investigate the effect of PRMT1 on cell growth and clone formation in gastric cancer cells,by the proliferation assay and clone formation assay were used to determine the growth curves.Transwell assay were used to detect the migration and invasion abilities in gastric cancer cells.Invasion-related protein were detected by Western blot.EMT marker protein were detected by Western blot.Expression levels of Hippo signaling related protein were examined by Western blot.Results:The expression levels of PRMT1 protein were higher in AGS and MGC803 cells than that in BGC823 and SGC7901 cells.In AGS and MGC803 cells transfected by sh-PRMT1 the protein and mRNA expression of PRMT1 was efficiently down-regulated.In BGC823 and SGC7901 cells transfected by Flag-PRMT1 the protein and mRNA expression of PRMT1 was strongly up-regulated.PRMT1 knockdown enhances the ability of proliferation in AGS and MGC803 cells.Contrarily,PRMT1 up-regulated inhibits the abilities of proliferation in BGC823 and SGC7901 cells.Knockdowning PRMT1 inhibits the abilities of migration and invasion and reduces the invasion-related levels in AGS and MGC803 cells.However,overexpressing PRMT1 enhances the abilities of migration and invasion and increases the invasion-related levels in BGC823 and SGC7901 cells.Knockdowning PRMT1 increases epithelial markers protein expression and downregulates expression of mesenchymal markers in AGS and MGC803 cells,whereas PRMT1 overexpression reverses these changes in BGC823 and SGC7901 cells.PRMT1 silencing promotes the phosphorylation of Lats1,and then induces YAP phosphorylation in AGS and MGC803 cells,while overexpression of PRMT1 down-regulates the phosphorylation of Lats1 and YAP in BGC823 and SGC7901 cells.Conclusion: The expression levels of PRMT1 protein in different gastric cancer cells were different.PRMT1 promotes the abilities of migration and invasion in gastric cancer cells,while it inhibites the ability of proliferation in gastric cancer cells.PRMT1 may protomes the epithelial-mesenchymal transition via Hippo signaling in gastric cancer cells.PRMT1 coordinates the epithelial-mesenchymal transition/proliferation dichotomy in gastric cancer cells.