Effects Of Integrin ?1 On Epithelialization And Contraction Of Mouse Skin Wound Healing In Vivo

Objective: To investigate the effects of integrin ?1 on skin wound healing by establishing mice skin wound model using integrin ?1 antagonist to antagonize integrin ?1 receptor.Methods: Fifty wide type 10-12 weeks C57 BL / 6J mice,the mice back skin were prepared.Using the random number table method to select the side which the sustained-release capsule osmotic pumpput inside,along the mouse back center line with the binaural ear root connection point as the starting marked the points 1.5 cm,4.0 cm and 5.0cm.Making a whole skin incision atthe selected side point 1.5 cm and implanting of sustained-release capsule osmotic pump,sutured incision.Experimental group pre-loaded integrin ?1 antagonist andthe control group pre-filled saline.Two days after operation,the diameter of 8 mm round whole layer skin was cut among the square area of 4.0 cm and 5.0 cm,each mouse was kept in a single cage and recorded.Specimens was harvested on the 3,5,7 and 14 day.The wound healing was analyzed by general observation and histological examination.Data were processed with t-test.Results:Recording the progress of wound healing,the observed healing time of the experimental group was slower than that in the control groupat each observed days.The average healing rate of the wound healing experimental group was(95.43 ± 1.13)% at 14 th day after operation,which was significantly lower than that of the control group(99.60 ± 0.402)%,the difference was statistically significant;HE staining showed that the epithelium of the experimental group was thinner and the granulation tissue was less,the edema of the experimental group was obvious,the infiltration of inflammatory cells was less,and the keratinocytes were accumulated on the margin which migrate to the center slowlycompared to the control groupat 3rd day.While the control group had a mild edema degree and the number of inflammatory cells was relatively large.The keratinocytes were distributed from the center of the wounded margin to the wedge.At 14 th day,the two groups new epithelium thickness test experimentalgroup(11.66 ± 0.38)were significantly lower than those in the control group(6.89 ± 0.05)um,the difference was statistically significant(P <0.05).Experimental groupthe expression of integrin?1 in the normal skin tissue around the wound was significantly lower than that in the control group at 3thday after immunofluorescence detection,the difference was statistically significant.Masson staining showed that collagen deposition in the experimental group was significantly lower than that in the control group at 14 th day,and the difference was statistically significant.The expression of integrin?1 in the wound was detected by immunohistochemical staining,suggesting that the expression of integrin?1 at the early 3rd day and 5th day experimental groupwas low,while the control group was highly expressed and was wedge-shaped at the margin.At 7th day and 14 th day,the expression of integrin?1 increasedbut the difference was not obvious;The expression of myofibroblasts in granulation tissue of ?-SMA showed that the expression in experimental group was significantly higher than that in the control group at 3rd day,5thday and 7th day,but the difference was not obvious at 14 th day.Ki67 staining was used to detect the proliferation of the cells.The results showed that the experimental group had less staining cells and less proliferative activity than the control group at 3rd day.Western blot was used to detect the expression of integrin ?1 in the wound.The results showed that the expression of integrin?1 in the wound experimental group was significantly lower than that in the control group at 3rd day and 5th day,and the difference was statistically significant.The expression of integrin ?1 at 7th day and 14 th day were increased,but the difference between groups was not obvious.Conclusion: The integrin ?1 antagonist is effective in subcutaneous sustained release administration,which can inhibit the migration of keratinocytes during the wound repair process,slow down the process of epithelialization of the wound,hinder the early systolic process and reduce the proliferation and metabolism of the wound cells.