The Mechanism Research On Anti-obesity Efficacy Of Pu-erh Tea

Puer tea is protected by geography,according to the different production process and quality,can be simply divided into Raw Puer tea(Raw)and Riped pPuer tea(Ripe).The present study evaluated the anti-obesity activity of aged Raw and aged Ripe to explore the mechanism of anti-obesity in HFD-induced obesity,it were investigated from the animal level and cell level,the obesity rat model and 3T3-L1 adipocyte model was used to investigate the inhibitory effect of extract of Raw and Ripe.Western blot and immunohistochemistry were used to investigate the anti-obesity effect of extract of Raw and Ripe.During nine weeks of continuous gavage,rats in the model group had a higher body weight than other groups.There was no significant difference between the groups in intake of drinking water.After dissection,white fat index and liver index were compared.and observed by H&E staining,the accumulations of white vacuoles and lipid droplets are showed that Raw and Ripe effectively reduced the fat mass of obese rats.It was observed that the phosphorylation of AMPK in the liver induced the phosphor-ylation of ACC and the ratio of P-AMPK/AMPK and P-ACC/ACC was significantly increased in the Raw pu-erh tea and Ripe pu-erh tea.At the same time,the activity of CPT-1 was increased,thereby significantly inhibiting the expression of FAS.In particular,in the Raw,the phosphorylation of AMPK was extremely marked,and the phosphorylation of ACC was stimulated to suppress the expression of FAS.All these indicate that Raw and Ripe may use the metabolic genes of this pathway to reduce HFD-induced abnormalities in fat metabolism,and do not rule out the regulation of other genes,but at least in part regulate the expression of certain genes through this pathway.Meanwhile,it was observed that the Raw and Ripe had effects on the inflammatory cytokines IL-1? and iNOS,thereby indirectly protecting the liver function and energy metabolism.There was no significant difference between the two groups.The 3T3-L1 preadipocytes were induced to differentiate using the induceddifferentiation medium I and II and the basic culture fluid for 8 days.The oil red O staining experiment was used to induce the increase in the number of lipid droplets in the differentiated adipocytes,and the small lipid droplets were fused to larger lipid droplets increase the volume of lipid droplets,indicating that 3T3-L1 preadipocytes have successfully induced differentiation into mature adipocytes.The up-regulation of genes that inhibited adipogenesis was observed in the Raw and Ripe,and the down-regulation of the synthetic fat genes was not concentration dependent.The expression of several related proteins showed that both Raw and Ripe have upregulated PAMPK and CPT-1,while down-regulating expression of FAS and inactivating ACC by increasing phosphorylated ACC(P-ACC)but not down-regulated in a dose-dependent manner,and there is not much connection between the two,suggesting that although the intervention effect of the Raw pu-erh tea and Riped pu-erh tea on lipid droplet formation in mature cells is affected by AMPK,it does not reduce intracellular lipid droplets through the same fatty acid oxidation pathway as the liver.The formation may be influenced by other proteins associated with AMPK.Through analysis of WB,the expression levels of C/EBP? and PPAR? were down-regulated in both the Raw and Ripe,while PPAR? decreased the expression level in a dose-dependent manner.Consistently,it was suggested that AMPK may be involved in the formation of intracellular adipogenicity interventions by Raw and Ripe.Through the intervention of 3T3-L1 preadipocyte proliferation and induction of differentiation into mature cells,Raw group can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes better than that of Ripe group.