A Bioinformatics Analysis Of Differentially Expressed Proteins In Obese Polycystic Ovary Syndrome Based On Quantitative Proteomic

Objective:Polycystic ovary syndrome(PCOS)is one of the most common endocrine and metabolic disorders in puberty and reproductive age and women,the incidence rate can be 5% to 10%.PCOS is a complex multigenic disorder and the pathogenesis of this syndrome remains largely unknow.Obesity,as one of the main clinical signs of PCOS,about 30% to 75% of patients with PCOS are overweight or obese,and adipose cells play an important role in the regulation of endocrine system.Therefore,the pathogenesis of PCOS patients with obesity and normal weight may be different.Isobaric tags for relative and absolute quantitation technique(iTRAQ)is a new quantitative proteomics technique,we use the iTRAQ technique to screen the differentially expressed proteins associated with PCOS in obese or non-obese,to explore the relationship between the pathogenesis of PCOS and the differential protein,and to provide more clues for exploring the pathogenesis,and to look for diagnostic markers related to PCOS.Methods:From January2017 to May2017,A total of 64 gynecological outpatients and healthy women were selected in the Third Affiliated Hospital of Guangzhou Medical University,including 16 cases of non-obese PCOS group and 16 cases of obese PCOS group;16 cases of healthy women and 16 of obese-healthy women.The serum of each group was collected,after being removed from the high abundance protein,the filtrate was quantified,dissolved and degenerated,reduced and alkylated,trypsin digested,and then identified with iTRAQ,coupled with 2DLC-MS/MS technology to quantitate and identify the proteins,and analyzed with bioinformatics tools finally.Results:The screening and identification revealed,93 proteins were differential expressed in non-obese PCOS,and protein which expression is increased by the top two fold change values is SPTBN4、CAPN2.179 differential expressed proteins in obese PCOS,and protein which expression is increased by the top two fold change values is KIF15、CAPN2.Compared with non-obese PCOS,67 proteins were differential expressed in obese PCOS,KIF15 is the differentially expressed protein which expression is largest increased.Compared with obese-control group,10 proteins were differential expressed in obese PCOS,KIF15 is also the differentially expressed protein which expression is largest increased.According to cellular component analysis,the differentially expressed proteins in obese PCOS and the non-obese PCOS were mainly exist in extracellular space;According to the molecular function analysis,the obese PCOS and the non-obese PCOS were mainly involved in protein binding.According to biological process analysis,the obese PCOS and the non-obese PCOS were mainly participate in responding to wounding and defending response.The KEGG pathway analysis showed that: the non-obese PCOS group was involved in 70 pathways,and the obese PCOS group was involved in 158 pathways,reflecting a different mechanisms between non-obese PCOS and obese PCOS.The most significant signaling pathway involved in the two groups was complement and coagulation cascades.Compare with the non-obese PCOS and the obese-control,the differential expressed proteins in obese PCOS were mainly involved in abnormal glucose metabolism.Conclusion: 1.We have successfully identified a large number of differentially expressed proteins using a proteomics approach based on iTRAQ technology.2.The differentially expressed proteins of PCOS were mainly involved in proteolysis,defense response,response to wounding,protein activation cascade,acute inflammatory reponse and the complement and coagulation cascades,which confirming the low grade chronic inflammation in PCOS.There were 158 pathways involved in obesity PCOS group,but only 70 were involved in non-obese PCOS group,suggesting that PCOS and non-obese PCOS have different regulatory mechanisms.KIF15 is a differential protein associated obese PCOS.CAPN2 could be served as a new biomarker for PCOS screening.