LncRNA ANCR Down-regulation Promotes TGF-?1-induced EMT And Metastasis In Breast Cancer

Epithelial-Mesenchymal Transition(EMT)is one of the key steps in the development of cell plasticity,differentiation,cancer cell migration and tumor metastasis.TGF-?1 is a pluripotent factor that can affect various physiological and pathological processes such as cell proliferation,differentiation,and immune regulation,and TGF-?1 can induce EMT.Therefore,TGF-?1-induced EMT is often used as a classic model to study changes in EMT-related factors.More and more evidence shows that long noncoding RNA(lncRNA)plays a key role in the process of EMT and tumor metastasis.However,there are still limited reports on the association of TGF-?1-lncRNAs-EMT.In previous studies in our laboratory,we found that lncRNA ANCR is involved in TGF-?1-induced EMT.In the process of EMT induced by TGF-?1 in breast cancer cells,TGF-?1 inhibits the expression of ANCR by decreasing the acetylation levels of H3 and H4 in the ANCR promoter.However,what role ANCR plays in TGF-?1-induced EMT and the mechanism through which ANCR functions is still unclear.This study found that ANCR as a new TGF-?1 downstream molecule can inhibit TGF-?1-induced EMT by reducing the expression of RUNX2,thereby inhibiting breast cancer cell migration and invasion,as well as breast cancer metastasis.Further studies found that there is a negative correlation between ANCR and RUNX2 expression in a variety of breast cancer cell lines and collected breast cancer tissue samples.These results suggest that ANCR may be a potential prognostic biomarker for breast cancer patients and a new target for anti-breast cancer metastasis therapy.