The Mechanism Of MicroRNA149-3p In Enteritis

As the lower part of the digestive system,the large intestine is an important part of the human digestive system and plays an important role in maintaining the normal internal environment of the human body.In the human body,the large intestine can further absorb fecal moisture,electrolytes and other substances.The inflammation or damage of the large intestine can affect human health.The types of enteritis that often appear are colitis and proctitis,and these diseases are harmful to the human body.MiR-149-3p is a small-molecule RNA whose main function is to bind to the 3 ' UTR or 5 ' UTR in the target mRNA,thereby regulating protein translation and degradation of mRNA.At present,the research on mRNA is more and more in-depth,and more research focuses on the combination of miRNA and cell signaling pathway.The main research in this experiment is the NF-?B signaling pathway.The NF-?B signaling pathway is a classical signaling pathway.Many studies have found that NF-?B signaling pathway is closely related to a variety of inflammations and cancers.In our study,it was also found that the inhibitory effect of miR-149-3p on enteritis is closely related to the NF-?B signaling pathway.In the experiment,we mainly studied the role of miR-149-3p in enteritis in animal experiments and cell experiments.In animal experiments,we mainly used mice to conduct experiments.DSS was used to induce intestinal inflammation in mice.A mouse enteritis model was constructed and its large intestine tissue was removed.Real-time quantitative PCR was used to detect the expression of inflammatory factors in mouse tissues.It was found that the expression levels of inflammatory cytokines IL-2,IL-8,TNF-a,and MCP-1 in the experimental group were significantly higher than those in the control group.After H$E staining,miR-149-3p-+/-was found.The mice were more severe and more sensitive to DSS-induced inflammation of the large intestine tract than WT wild-type mice.In addition,we measured the phenotypic characteristics of mice,and the length of the large intestine of the miR-149-3p-/-mice in the treated group was significantly shorter than that of the WT wild-type mice,and the percentage of weight loss in the mice of the miR-149-3p-/-DSS group.Significantly higher than wild-type DSS mice,that is miR-149-3p-/-mice are more sensitive to DSS-induced enteritis.In cell experiments,we used the human colon cancer cell caco2 to conduct an experimental study.Real?time quantitative PCR was used to detect the expression of inflammatory factors in the cells.The miR-149-3p treatment group inhibited COX-2,IP-10,iNOS,and MCP-1.Expression of IL-1? and TNF-?.After induction with TNF-?,the expression levels of these genes were significantly reduced.Western Blot experimental study found that miR-149-3p can inhibit the phosphorylation of Ir?B? The luciferase assay found that the miR-149-3p nucleic acid mimics-treated group was able to inhibit the activity of the NF-?B reporter plasmid.In summary,it can be concluded that miR-149-3p can inhibit enteritis by inhibiting the NF-?B signaling pathway.