Effect And Mechanism Of LLEST Reducing Glucose Uptake In Acute Myeloid Leukemia Cells

Objective1.To investigate the effects of long non-coding RNA-LLEST(Lnc RNA-LLEST)on the glucose uptake in K562,SHI-1 and NB4 cell lines.2.To study the effects of LLEST on glucose transporter 10(GLUT10)levels in K562,SHI-1 cell lines.3.To investigate the difference levels of LLEST and GLUT10 in AML-M2 patients and anemia patients.Methods1.After K562 and NB4 LLEST overexpressed stable cell lines and SHI-1 knock down stable cell lines were selected,the uptake rate of Fluorine-18-fluorodeoxyglucose(18F-FDG)were detected in these cells.2.The relationship between LLEST and GLUT10 was observed by q RT-PCR and Western Blot.3.LLEST and GLUT10 levels were detected by q RT-PCR in bone marrow mononuclear cells(BMMC)from AML-M2 and control samples.Results1.LLEST inhibited the uptake of 18F-FDG in vitro.After induced by low glucose,the uptake of 18F-FDG was measured by ?-meter.Results showed that 18F-FDG uptake rate decreased in LLEST overexpressed K562 and NB4 cell lines.While the 18F-FDG uptake rate increased in LLEST knock down SHI-1 stable cell lines.2.LLEST inhibited glucose uptake by inhibiting GLUT10 expression.To see how LLEST affected the glucose uptake,q RT-PCR was measured in LLEST overexpressed K562 and in LLEST knock down SHI-1 stable cell lines.Results showed that the level of GLUT10 was down when LLEST was overexpressed in K562 cell lines;while knockdown LLEST in SHI-1 cell lines,the level of GLUT10 was up.Western Blot assay revealed that in K562 cells when LLEST was overexpressed,GLUT10 levels decreased.3.Compared with anemia patients,GLUT10 was increased in bone marrow cells of M2 patients,while LLEST was decreased.Bone marrow mononuclear cells from AML-M2 and anemia patients were collected.q RT-PCR results revealed that GLUT10 was increased in bone marrow cells of M2 patients while LLEST was decreased,compared with anemia patients.Conclusions1.LLEST could affect glucose uptake of leukemia cells in vitro.2.LLEST could affect glucose uptake through inhibiting GLUT10 expressed,but whether it played a leading role remained to be further verified.3.LLEST also inhibited GLUT10 expression in clinical samples.