| Objective In recent years,traditional Chinese medicine has played an active role in the treatment of bone marrow suppression after chemotherapy,and has attracted more and more attention and research.Chinese traditional Chinese medicine can promote stem cell proliferation and differentiation through different mechanisms,which can slow down the aging of stem cells.Is put forward according to the early stage of the team "stem cell has the congenital essence of attribute,is the essence of nature in the form of the cellular level",this topic will look at cisplatin chemotherapy in the stem cell lesions seen,discusses the regulation of bone marrow stem cells of kidney method antagonism effect mechanism of bone marrow suppression caused by chemotherapy,provide choice for clinical effective prevention and treatment of radiation and chemotherapy lead to bone marrow suppression party basis and train of thoughtIn the early stage of the research study,on the basis of selected between bone marrow mesenchymal stem cell proliferation has a promoting effect of single taste traditional Chinese medicine,Chinese medicine monomer,analyzing their protective effect of MSCs damage caused by cisplatin chemotherapy to explain their antagonism caused bone marrow suppression mechanism provides the experimental basis,also for the "pure" stem cell theory provides a sufficient scientific basis..Methods 1.cell experiment(1)the whole bone marrow adherent wall method was used to separate and cultured bone marrow mesenchymal stem cells.(2)CCK-8 method was used to screen single Chinese herbs and monomers with protective effects on cisplatin,and to study their optimal time and concentration.(3)after the induction of MSCs 72 h with the concentration of cisplatin in the concentration of 12.97,MSCs(MSCs IC50)and THSG,the cell apoptosis was measured by flow cytometry.Osteosynthesis and lipogenic differentiation were detected,and real-time PCR was used to detect m RNA expression of relevant cytokines.2 Experiments on animals(1)biological information data mining the expression of the bone marrow differential gene in cisplatin.(2)model: according to the preclinical evaluation method of cytotoxic anti-tumor drugs,100 g SD male rats were selected to be injected with cisplatin in the tail vein of 4.5mg/kg to replicate the bone marrow suppression model.(3)grouping: the experimental rats were randomly divided into 3 groups,with 10 in each group,and divided into normal group,model group and THSG group.(4)drug delivery methods: THSG groups: the first day at 8 a.m.in accordance with 4.5 mg/kg iv cisplatin tail one-time bone marrow suppression model,4 PM diphenylethylene glycosides(concentration of 1 x 10-4mol/L)to fill the stomach,to fill the stomach once a day,7 days in a row.Model group: at 8 o 'clock on the first day,the injection of cisplatin at 8 o 'clock at 8 o 'clock was followed by the injection of cisplatin,which was used to control the mold,and the daily saline was used for 7 days in a row.The control group was treated with saline for 1 to 7 days.(5)monitoring of related indicators.(1)Blood routine: normal group,model group,and experimental group were tested for routine blood test on the second day,5 days and 8 days after eyeball.(2)The cell cycle was detected by flow cytometry and the detection of CD105 positive cells in bone marrow stem cells.(3)The cell cycle Cdkn1 a Ccne1 gene expression was detected by real-time PCR.Results1.Cell experiment(1)After 72 hours of co-culture with cisplatin,the MSCs were 25% and 1 x 10-4mol/L THSG and cisplatin.The proliferation was significantly promoted(P<0.05).(2)A total of 25% of the drug serum,1 x 10-4mol/L THSG and cisplatin were cultured in MSCs72 hours respectively,which could slow the apoptosis of cisplatin induced MSCs early cells(P<0.05).(3)In addition,25% of the drug serum,1 x 10-4mol/L THSG has a certain protective effect on cisplatin induced MSCs osteoblast differentiation,and can promote the expression of ALPL and OGN of osteogenic related markers.25% of the serum,1 x 10-4mol/L THSG of the drug has certain protective effects on cisplatin induced MSCs,and can promote the expression of LPL and CFD of lipid-related marker genes.2.Animal experiment(1)In the 8th day after chemotherapy,1 x 10-4mol/L THSG had a significant improvement in peripheral blood leukocyte count(P<0.05),and the improvement of erythrocyte and platelet was not obvious(P>0.05).(2)8 days after chemotherapy compared with control group,model group cell cycle S phase proportion increased significantly,but THSG group compared with control group,there was no significant difference,THSG group compared with model group significantly lower proportion of cell cycle S period,there are significant differences(P < 0.05);Compared with the control group,the THSG group and the model group showed significant differences(P<0.01),while the THSG group and the model group showed significant differences between the CD105 cells(P<0.050).(3)Compared with the control group,the expression of CDKN1 A gene in the THSG group and the model group was significantly different(P<0.05)compared with the control group in the fifth day after chemotherapy,and the expression of CDKN1 A gene was significantly different in the THSG group and the model group(P<0.05).Compared with the control group in the 8th day after chemotherapy,CCNE1 gene expression was up-regulated in THSG group,with significant difference(P<0.05),and the expression of CCNE1 gene was up-regulated in the THSG group and the model group,with significant difference(P<0.05).Conclusion1.The proliferation of MSCs can be significantly promoted after the cultivation of MSCs72 hours with the drug serum and 1x10-4 mol/L THSG and cisplatin.It can slow the apoptosis of cisplatin induced MSCs early cells.It has a certain protective effect on cisplatin induced MSCs osteosynthesis and lipid differentiation,and can promote the expression of osteogenic,lipid-related marker genes ALPL,OGN,LPL and CFD.2.10-4mol/L THSGcan contribute to the proliferation of bone marrow stem cells in bone marrow suppression model,reduce the cell cycle S phase retardation,improve after chemotherapy peripheral white blood cell count,its mechanism may be related to THSG inhibition of bone marrow suppression model CDKN1 A gene expression,raise CCNE1 expression of related genes... |
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