Experimental Study Of Ecdysterone Intervention On The Injured Chondrocytes Of Rats

ObjectivesTo investigate the effect of ecdysterone(EDS)on the proliferation and apoptosis of injured chondrocytes and explore its mechanism.Methods1.The cartilage tissues of the articular cartilage of the lower femur of SD rats were isolated by surgery.2.Primary cells were obtained by co-digestion with 0.25% trypsin and 0.2% type II collagenase.3.The cells were identified by morphological observation,toluidine blue staining and type II collagen immunofluorescence staining.4.The injured chondrocyte model was constructed using cell scratches to simulate the biological changes of cartilage injury.5.The optimal drug concentration for EDS to act on damaged chondrocytes was screened,and then this concentration would be used for subsequent experiments.6.The proliferative activity of cells in each group was detected by MTT,and to study the effect of EDS on the proliferation of injured chondrocytes.7.The apoptosis rate of cells in each group can detected by Flow cytometry,then studied the effect of EDS on the apoptosis of the injured chondrocytes.Results1.The primary chondrocytes with high activity and purity were successfully obtained in this experiment.2.The optimal concentration of EDS affecting on the injuring chondrocytes was 3μmol / L.3.Compared with the control group,the proliferation activity of the injured cells was significantly decreased(P < 0.05);compared with the injured group,the proliferation activity of the cells in the EDS intervention group was significantly increased(P < 0.05);there was no significant difference in proliferation activity between EDS intervention group and control group(P > 0.05).4.Compared with the control group,the apoptosis rate of the injured group was significantly increased(P < 0.05);compared with the injured group,the apoptosis rate of the EDS intervention group was significantly decreased(P < 0.05);there was no significant difference in the cell apoptosis rate between EDS intervention group and control group(P > 0.05).ConclusionsEDS promotes the proliferation of injured chondrocytes and inhibits the apoptosis of injured chondrocytes,suggesting that EDS has a protective effect on chondrocytes,which could provide experimental data and theoretical basis for the repair of sports cartilage injury.