Preparation And Immune Activity Of Buccal Of Polysaccharide From Ganoderma Atrum

Ganoderma atrum is a basidiomycetaceae,commonly known as "Ganoderma".It has a long history in our country and other Asian countries.Black ganoderma is the ultimate in Ganoderma,and its therapeutic effect on human consumption and medicine is much higher than that of other kinds of ganoderma.In order to better development and utilization of black Ganoderma resources,our laboratory to black Ganoderma atrum do a lot of basic research work,the existing results show black polysaccharide of Ganoderma atrum fruiting body composition has significant immunomodulatory,antitumor,anti-aging,fall blood sugar,prompt black Ganoderma atrum polysaccharide of promising prospects in exploitation and utilization.At present,there are a lot of dark Ganoderma products in the market,but there are very few researches on the development of polysaccharide related products.Therefore,this paper explores black-black Ganoderma atrum polysaccharide contents of Ganoderma atrum polysaccharide product development,research the black Ganoderma atrum polysaccharide contents of the craft formula,through the establishment of immunosuppressive mice model,and discusses black Ganoderma atrum polysaccharide contents of the regulation of immune function in mice model.The main research contents and results are summarized as follows:(1)The process formulation of polysaccharide in black Ganoderma polysaccharide was established and the quality control standard was established.Using single factor and orthogonal test method and combined with sensory evaluation black Ganoderma atrum polysaccharide lozenges experiment to obtain the optimum formula is: 40% black Ganoderma atrum polysaccharide,25% mannitol and 25% xylitol,2% microcrystalline cellulose,2% sodium carboxymethyl cellulose,2% magnesium stearate,2% of cross-linked polyvinylpyrrolidone,2% citric acid.According to the standard of Chinese pharmacopoeia in 2015,the standard for the establishment of polysaccharide tablets of black Ganoderma is: hardness in the range of 40-60 N;The weight difference is within the range of less than 5%;The disintegration time is 10-30 min;There are no e.coli and other pathogens in microbial limit detection.(2)To study the immunological function and intestinal health regulation of the polysaccharide tablets of Ganoderma atrum.By intraperitoneal injection of cyclophosphamide,immunosuppressive mice model was established,to Cheng Mo lavage black g Ganoderma atrum polysaccharide in mice buccal tablet solution for a week,doses are: low dose(25 mg/kg b w),middle dose(50 mg/kg b w)and high dose(100 mg/kg w b).The results showed that the gastric low,medium and high dose of black Ganoderma atrum polysaccharide lozenges solution can make weight rebound in immunosuppressive mice,increase the thymus index in mice,increased spleen lymphocyte subsets CD3 +,CD4 +,CD8 + and CD4 + / CD8 + ratio,promote the secretion of spleen lymphocytes IL-4 and NO increase in serum IL-2 and TNF-? content,improve the body's immune function.In addition,to fill the stomach high-dose black Ganoderma atrum polysaccharide lozenges can significantly increase colon in mice feces SCFAs(acetic acid and butyric acid)content,lower colonic fecal p H value,which to some extent help intestinal health.The above results indicated that the polysaccharide tablets developed in this study had the effect of enhancing immune function and promoting intestinal health to some extent.