The Effects Of Glucose And Lipid Metabolism Of The Key Bacteria Isolated From Uighurs And Kazaks With Type 2 Diabetes Mellitus In Mice

Objective: 1.To isolate Bacteria A and Bacteria E from fecal samples of Uygurs,Kazaks with type 2 diabetes in Xinjiang.2.To observe the effect of Bacteria A,Bacteria E on glucose and lipid metabolism of C57 mice.Methods: 1.Fresh fecal samples of Uygurs,Kazakhs with type 2 diabetes mellitus were collected to isolate Bacteria A,Bacteria E by using specific media;micro-biochemical reaction were adopted for preliminary identification;specific primer was used to amplify the DNA of the strains;and fully automated bacterial identification system was used for strain indentification.2.Bacteria A,Bacteria E was inoculated into the mice,and the colonization force was detected by CM-Dil labeling.3.Male C57BL/6 mice were divided into four groups: common chow diet-fed animals(Normal),common chow diet-fed animals with Bacteria A,high fat diet and high fat diet with Bacteria A,Use the blood of tip of the tail to detect the level of fasting glucose(FBG),random blood glucose(GLU),total cholestero(TC),Triglyceride(TG),Oral glucose tolerance test(OGTT)and Insulin tolerance test(ITT).Bacteria E is consistent with Bacteria A.Reslut: 1.The isolated strain was identified as Bacteria A by fully automated bacterial identification system,The desired fragment was obtained by the amplification.The Bacteria E was described as Bacteria identification manual.2.Fluorescent markers were detected in the bacteria isolated from mouse feces.3.At 3 weeks,Compared with HFD mice,the body weight of HFD+Bacteria A significantly increased;At week 8 the content of GLU,FBG,TC,TG were significantly increased(p<0.05,p<0.001,p<0.05);Bacteria A,HFD+Bacteria A group appear impaired glucose tolerance since week 4,the AUC of glucose in OGTT were significantly increased;Bacteria A,HFD+Bacteria A group appear impaired glucose tolerance since week 8,the AUC of glucose in ITT were significantly increased.4.At 4 weeks,Compared with HFD mice,the body weight of HFD+Bacteria E significantly increased;At week 12 the content of GLU were significantly increased(p<0.001);At week 8 the content of TC,TGwere significantly increased(p<0.001,p<0.05);Bacteria E,HFD+Bacteria E group appear impaired glucose tolerance since week 4,the AUC of glucose in OGTT were significantly increased;Bacteria E group appear impaired glucose tolerance since week 8,the AUC of glucose in ITT were significantly increased;HFD+Bacteria E group appear impaired glucose tolerance since week 4,the AUC of glucose in ITT were significantly increased.Conclusion: Bacteria A and Bacteria E isolated from fecal samples of Kazakhs with type 2 diabetes mellitus,the colonization was successful and increased the weight of mice;GLU increased;HFD+Bacteria impaired glucose tolerance,insulin sensitivity.