| Cancer has been the major season to injury human healthy because of the unlimited reproduction and easy metastasis.The traditional cancer treatment of chemotherapy,radiotherapy and surgery have not the ability to cure cancer as well as no side effects,efficient treatment effect and painless.Therefore,nanoparticles have been utilized to cancer therapy because it can reduce toxic side effects effectively and prevent tumor metastasis and recurrence.On the other hand,the diverse structure can help the nanoparticles to realize that decrease the dose of drug by the targeting molecular,then the nanoparticles can use the heat energy to kill temperature-sensitive cancer cells by the photothermal conversion performance and produce ROS to ruin cancer cell by the photosensitive molecules.Meanwhile,the nanoparticles can be a diagnosis agent to guide cancer therapy by magnetic resonance imaging,photoacoustic imaging,ultrasound imaging and fluorescence imaging etc.In the end,nanoparticles have been used in anti-tumor applications and have solved the problem of large toxic and side effects of traditional treatment methods.They also solved the requirements of tumors for precise treatment mediated by imaging technology and built an integrated treatment platform for diagnosis and treatment.Metal-organic frameworks have caused wide concern due to the large specific surface area,porosity,and easily modified structure compared with traditional nanoparticles.It has been used to gas adsorption,catalysis and biomedicine etc,for instance,the large pore size characteristics suitable for encapsulating drugs exhibit a high loading rate;the relatively unstable coordination bonds between the metal and the ligand make it biodegradable;the use of post-synthetic modification can make it more portable.The response nanoparticles have successful solved the problems of drug release,and the intelligent control release is the key to be functional.As we all known the pH and temperature are two signal molecules that are often used as a response in release process of nanocarrier.Especially,nanoparticles use their instability at lower pH to release drug molecules that can be used in anti-tumor applications due to the tumor's acidic environment.In addition,temperature as a signal is often designed as a responsive signal molecule,and using nanocarriers to release the drug at different temperatures with the changes of structure.Enhance,the responsive nanoparticles can increase the efficiency of drugs and kill cancer cells effectively.This article aimed to establish a MRI guided nanocarrier which can release anti-cancer drug by the pH and temperature response,and combine with photothermal and photodynamic therapy.Then by using DOX as target drug to explore the MRI-guided cancer therapy and evaluate the tumor elimination.Experiment content:1,The PCN-600 nanoparticles were abtained by solvothermal methord,then used the dopamine to modify it by amide bond.The PCN@PDA nanoparticles would improve the absorption of NIR light and be an excellent nano-drug to kill cancer cells.Secondly,by using the photosensitivity of the ligand in the metal-organic framework material,it will produce singlet oxygen under the irradiation of near-infrared light,achieving the dual role of photothermal and photodynamic cancer therapy.2,The targeting drug of DOX was uploaded by MOFs to prove the porous,and we found that the DOX had been uploaded about 80%.For others,the versatile nanopartices could release DOX efficiently due to the dual-modal response of pH and temperature after the coating of PDA,and achieve the controlled chemotherapy under the microenvironment of tumor.3,In vitro and in vivo evaluated the ability of tumor elimination and imaging.First of all,we determined the biosafety of the PCN@PDA,then studied the cellular uptake and specific killing effect at the cell level,evaluated the mechanism of action of the material and the cell,and finally assessed the anti-tumor effect and tumor imaging of the material at the animal level.To explore whether the material could have no significant effect on other mouse tissues based on the effective killing of tumor cells. |
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