Studying The Function Of MiR-17?92 In Plasma Cell Differentiation By Ribosome Profiling

Plasma cells are the antibody secreting cells of the immune system.They differentiate from activated B cells and are the cellular basis of humoral immunity.B cell activation,proliferation,and plasma cell differentiation are controlled by multiple regulatory pathways,whose elucidation is of fundamental importance for the design of better vaccines and therapies aimed to treat autoimmune diseases.Recent studies have shown that microRNAs(miRNAs)play critical roles in the immune system.The miR-17?92 family miRNAs are essential for B cell development and central tolerance,but it remains unclear whether these miRNAs also control plasma cell differentiation.My study revealed that B cell-specific deletion of the miR-17?92 family miRNAs severely compromised B cell proliferation and plasma cell differentiation.As our previous studies showed that miR-17?92 controls its target gene expression mainly at the translational level,we employed ribosome profiling to identify target genes regulated by miR-17?92 during plasma cell differentiation and found that metabolic pathways were significantly dysregulated upon the loss of miR-17-92 family miRNAs.These results suggest that miR-17?92 controls B cell proliferation and plasma cell differentiation through regulating metabolism.