Designs,Syntheses And Applications Of Nano-drug Carrier System Based On Polydopamine

Cancer has always been a nightmare for humans.Although there are various anti-tumor drugs on the market at present,chemical drugs inevitably have centain side effects.To improve the efficiency of tumor therapy and reduce the side effects of chemical drug,there are some general methods.For example,drugs with different mechanism was used to combination therapy,drug carriers were used to targeted transport drugs by utilizing the EPR effect of tumor tissue and the unique microenvironment of tumor cells,and photothermotherapy and chemotherapy was combined.PDA(polydopamine)is a kind of biological material with good biocompatibility,rich active group on the surface and photothermal properties.Based on natural features of PDA and the PER effect of tumor tissue,PDA was designed to antitumor's drug carriers with good application prospect.In this paper,three drug carriers are synthesized based on PDA,and their biological properties are studied.The main conclusions are as follows:1.Study on PDA-CMCS NPs drug delivery systemIn this paper,PDA-CMCS NPs were designed and synthesized with negative charge on the surface.It has a particle size of 140 nanometers.Experiment results show that PDA-CMCS NPs has excellent photo-thermal exchangeability and water solubility,and the anti-cancer drug DOX is loaded by electrostatic adsorption.The release profile's results showed that the drug release had obvious pH-response,cytotoxicity test results showed that the PDA-CMCS NPs had no significant toxicity to HL-7702 and Hep G2 cells in vitro,but the drug-loaded PDA-CMCS-DOX-DDP showed good results in inhibiting the activity of tumor cells.In the evaluation of anti-tumor effect in vivo,the combination of chemotherapy and photothermal(PDA-CMCS-DOX-DDP/PTT)not only showed the effect of effectively inhibiting the growth of solid tumor,but also showed that reduced the side effect of drugs.PDA-CMCS NPs as drug delivery carrier not only have the ability of the controlled drug release behavior,but also have the advantage to combine chemotherapy and hyperthermia,which has increased anti-cancer efficiency while reduced the toxic effects of anti-tumor drugs.2.Study on a multi-functional drug delivery systems based on Fe3O4 @PDAIn this paper,Fe3O4@PDA-PEG-FA and Fe3O4@PDA-TPGS were successfully prepared based on the core-shell structure of Fe3O4@PDA.The performance test results show that they have good magnetic responsiveness and photothermal conversion properties.The results of the simulated release showed that the drug delivery systems loaded with BTZ via boric acid have the ability of pH-responsive release in vitro.Fe3O4 @ PDA-PEG-FA has almost no toxicity to HL-7702 and Hep G2 cells.Fe3O4 @ PDA-TPGS showed little toxicity to HL-7702 cells and a certain toxicity to Hep G2 cells.The drug-loaded materials of Fe3O4 @ PDA-PEG-FA-BTZ and Fe3O4 @ PDA-TPGS-BTZ showed good toxic effect to Hep G2 cells,which proved that the Fe3O4 @ PDA-PEG-FA and Fe3O4 @PDA-TPGS as drug carrier not only have good biological compatibility,but also have the potential to combine target,photothermotherapy and chemotherapy.