Effects Of GINS2 On The Biological Behavior Of Human Nasopharyngeal Carcinoma Cell Line

Objective: To investigate the effects of GINS2(GINS complex subunit 2)on the biological behavior of human NPC(nasopharygeal carcinoma)5-8F and CNE-2Z cell lines.Methods: The shRNA targeting GINS2 lentiviral vector was transfected into human NPC 5-8F and CNE-2Z cells.The efficiency of transfection was observed under fluorescence microscope.Real-time quantitative PCR(qRT-PCR)was used to detect GINS2 expression at mRNA level.Western Blot was used to detect GINS2 expression at protein level.Celigo image cytometry was used to assess the growth ability of 5-8F and CNE-2Z cells after transfection.MTT assay was used to assess the proliferation ability of the transfected cells.Colony formation assay was used to detect tumorigenesis ability of the transfected cells in vitro.Flow cytometry was used to detect the apoptosis rate of the transfected cells.Results: GINS2-shRNA was successfully transfected into 5-8F and CNE-2Z cells.Each cell line was devided into two groups: shCtrl group and shGINS2 group.qRT-PCR and Western Blot showed that the expressions of GINS2 mRNA and protein were significantly inhibited in shGINS2 groups than in shCtrl groups.The results of the functional experiment in vitro were as follows.Celigo image cytometry revealed that the growth ability of the cells in shGINS2 groups was inhibited than shCtrl groups.The MTT assay showed that the proliferation ability in shGINS2 groups was also suppressed.Colony formation assay revealed that the colony number in shGINS2 groups was reduced.Flow cytometry showed that the apoptosis rate increased in shGINS2 groups than in shCtrl groups.Conclusion: In vitro experiments showed that the lentivirus-mediated GINS2 shRNA can inhibit the growth and proliferation,and promote the apoptosis in human 5-8F and CNE-2Z cells,which suggested that GINS2 may play an important role in the development of nasopharyngeal carcinoma.