| Objective:To detect the expression of P53,VEGF and ?-catenin proteins in liver metastases of colorectal cancer,and compare the age,sex and pathology of P53,VEGF,?-catenin protein expression and colorectal cancer liver metastasis(CRLM).The correlation between the clinical pathological factors,such as the degree of differentiation,clinical pathological stage,regional lymph node metastasis,depth of primary tumor invasion,and preoperative CEA levels,was analyzed for the correlation between the expression of P53,VEGF,and?-catenin proteins.Clinical diagnosis and treatment of colorectal liver metastases provide new ideas.Methods:Collected 60 paraffin-embedded specimens of colorectal liver metastases that were surgically removed and pathologically diagnosed between January2014 and January 2018,and examined P53 by immunohistochemistry(IHC).The expression of VEGF and ?-catenin protein in liver metastases of colorectal cancer.At the same time,the clinical and pathological data of this group of patients were collected.The expression of P53,VEGF and ?-catenin protein wasanalyzed between the clinicopathological features of this group of patients.Relationships;Spearman rank data correlation analysis was used to investigate the correlation between the expression of P53,VEGF,and ?-catenin proteins.RESULTS:1.P53 protein is mainly expressed in the cell nuclei of colorectal liver metastases,and the positive expression rate is 63.33%.The expression of P53 protein in liver metastasis of colorectal cancer was related to the presence or absence of regional lymph node metastasis and depth of primary tumor invasion(P<0.05),but related to gender,age,primary tumor location,tumor differentiation,preoperative There was no correlation between the level of CEA and the size and number of liver metastases(P>0.05).2.VEGF protein was mainly expressed in cytoplasm in colorectal liver metastases,and the positive expression rate was 71.67%.The expression of VEGF protein in liver metastases of colorectal cancer was related to the presence or absence of regional lymph node metastasis,preoperative CEA levels,and depth of primary tumor invasion(P<0.05),as well as gender,age,primary tumor location,There was no correlation between the degree of tumor differentiation and the size and number of liver metastases(P>0.05).3.The ?-catenin protein was mainly expressed in the cytoplasm of colorectal liver metastases,and the positive expression rate was 66.67%.The expression of ?-catenin protein in liver metastases of colorectal cancer was related to the presence or absence of regional lymph node metastasis and depth of primary tumor invasion(P<0.05),but related to gender,age,location of primary tumor,degree of tumor differentiation,There was no correlation between preoperative CEA levels and liver metastases size and number(P>0.05).4.In colorectal liver metastases,there was no correlation between P53 and VEGF protein expression(r=0.265,P>0.05).There was no correlation between P53 and ?-catenin protein expression(r=0.234,P>0.05).Positive correlation with ?-catenin protein expression(r=300,P<0.05).Conclusion:1.P53,VEGF and ?-catenin proteins are highly expressed in liver metastases of colorectal cancer and are associated with the presence or absence of regional lymph node metastasis and the depth of primary tumor infiltration.High expression of P53,VEGF and ?-catenin proteins Whether it is related to the mechanism of liver metastasis of colorectal cancer deserves further study.2.In colorectal liver metastases,VEGF is positively correlated with?-catenin protein expression,and both are related to the presence of regional lymph node metastasis and primary tumor invasion,suggesting that VEGF and?-catenin protein are involved in colorectal cancer There may be a synergistic effect on liver metastases. |
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