The Experimental Study On Abductor And Adductor:Comparative Study Of Morphology And Content Of Skeletal Muscle Glycogen

Objective:For the larynx,the normal contraction of the laryngeal muscles is the basis for the function of the larynx.The physiological functions such as laryngeal pronunciation,breathing and others have closely relationship the laryngeal muscles.Recurrent laryngeal nerve injury diseases have extremely complex pathogenesis,and play an important role in these mechanisms of laryngeal muscles.This paper took 13 cases of laryngeal constrictor,laryngeal abductor muscle to make anatomy,histology,ultra-microstructure,histochemistry and other aspects of the study and took advantage of morphometric methods to make comparative analysis to lay an experimental foundation for the follow-up study and the exploration of cause of laryngeal muscleMethod:1.Simulating the approach of head and neck surgery in five cadavers,which were proeessed by 10%formalin.dissect the cricoarytenoid muscle and lateral cricoarytenoid muscle.The paper observed and made records for the shape of the laryngeal cavity and also measured volume parameters.At the same time,it adopted the SLR camera(Canon 600D)and the macro lens(Canon 18-55ISII)were used to photograph the specimens image of the posterior sacral rotator and the lateral sacral muscles on a professional static radiography platform.2.The electron Microscopy studied the structural parameters of the posterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis of the 13 cases of the same specimen.The content of glycogen in theposterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis,of the same specimen was measured by anthrone method respectively.3.The laryngeal adductor muscle was taken from the posterior circoarytenoid muscle,and the abductor muscle was taken from the posterior circoarytenoid muscle.Immunohistochemical technique was used to identify 13 cases of the posterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis type I,type II muscle fibers,and to photograph under a microscope,the ImagePro Plus 6 images were used to analyze the compositional comparison of muscle fibers of the two groups through the circulation of coomputer.The HE staining technique was used to observe the fibrous structures of the posterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis in 13 cases of the same specimen.Result:1.The posterior circoarytenoid muscle is in the posterior mucosa and the abdominal shape is in the shape of fan.The muscles on the left and right sides are in a curved line.It arises from both sides of the posterior median ridge of the posterior plate of the cricoid cartilage and diverging distally.and the posterior circoarytenoid muscle of both sides tend to converge obliquely outwards,upwards,and forwards and stop behind the condylar cartilage of the same iliac cartilage.The musculi cricoarytaenoideus lateralis lateral sacral muscle is located on the anterolateral side of the condyle of the arytenoid cartilage.It starts at the upper edge of the cricoid arch of the left and right sides and stops in front of the condylar part of the arytenoid cartilage upwards,backwards,and inward.The average length of the posterior circoarytenoid muscle was 12.3mm±0.72 mm,in the middle 15.3mm±1.33 mm,and in the lower edge 20.3±1.55mm;the upper edge of the average width was 18.5mm±1.12 mm,the center was 15.2mm±0.74 mm,and the lower edge was 7.4mm± 1.45 mm;the upper edge of the average thickness is 2.3±0.54 mm,and the middle is 2.3mm±1.35 mm.The average length of the lateral sacral muscles was 18.4mm±1.45 mm in the upper part,12.3mm±1.34 mm in the middle,and 7.3mm±0.54 mm in the lower edge;15.1mm±0.49 mm in the upper edge of the average width,9.3mm±0.53 mm in the center,and and 4.2mm±1.56 mm in the lower edge;the upper edge of the average thickness is 2.3mm±1.72 mm and the middle edge is 4.3mm±1.56 mm.2.For the13 cases of posterior circoarytenoid muscle,its area density is 0.673±0.001826,volume density is 0.07246±0.001330,surface density is 0.35323±0.001481.For the 13 cases of musculi cricoarytaenoideus lateralis,its area density is 0.42±0.006083,volume density is 0.04792+0.000954,surface density is 0.17623± 0.001589.The mitochondrial area density,volume density and surface density of the posterior circoarytenoid muscle from the same specimen were all greater than those of the musculi cricoarytaenoideus lateralis,and the difference was extremely significant(P<0.05).After the measurement of the glycogen content of laryngeal muscle per 100 g,the posterior circoarytenoid muscle was 0.09438g±0.003228 g and musculi cricoarytaenoideus lateralis was 0.7477g±0.003059 g.there was significant difference between the groups(P<0.05)for the glycogen content,the glycogen content in posterior circoarytenoid muscle was higher than that the musculi cricoarytaenoideus lateralis.3.HE dyeing could see that the posterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis had transverse stripes and other structures.Measuring the compositional proportions of posterior circoarytenoid muscle and musculi cricoarytaenoideus lateralis I type fiber were as 75.5%± 4.2% and 22.5%±2.8% respectively.The proportions of type II fiber were 24.5%±4.2% and 77.5%±2.7 %respectively.Based on the comparison of the two groups,the proportion of type I fibers in the posterior circoarytenoid muscle was higher than that in the musculi cricoarytaenoideus lateralis,and the proportion of type II fibers was lower than that in the musculi cricoarytaenoideus lateralis.Conclusion:The adductor muscle of the larynx represented by the musculi cricoarytaenoideus lateralis,and the abductor muscle of the larynx represented by the posterior circoarytenoid muscle have great difference in the aspects of anatomy,ultrastructure,immunohistochemistry,and this difference is in accordance with the different functions of the two muscles in the body's throat,such as pronunciation,breathing,and protection of the lower respiratory tract.