The Involvement Of Rab27B On Invasion And Metastasis Of Human Breast Cancer Cell And The Intervention Mechanism Of Calycosin

Objective: To explore the effect of calycosin on invasion and metastasis of human breast cancer,as well as the involvement of Rab27 B in this action.Methods: Immunohistochemical analysis was performed to determine the different expression of Rab27 B in human breast cancer and the adjacent noncancerous tissues of 75 cases.Next,Recombinant lentivirus expressing the Rab27 B proteins was used to transfect human breast cancer cell line MDA-MB-231.Wound-healing and transwell assays were performed respectively to assess the value of Rab27 B overexpression on migration and invasion of MDA-MB-231 cells,and the effect of calycosin on migration and invasion of Rab27 B transfected or non-transfected breast cancer cells.Meanwhile,the real-time quantitative PCR and Western blotting were performed respectively to determine the effect of Rab27 B up-regulation on expression of VEGF in breast cancer cells,and calycosin-induced different levels of VEGF and MMP-9 in transfected or nontransfected MDA-MB-231 cells.Results: The expression levels of Rab27 B in human breast cancer tissues was obviously higher than in the noncancerous tissues(P<0.05);After transfection with recombinant lentivirus carrying the Rab27 B gene,the expression of Rab27 B in MDA-MB-231 cells was increased at the RNA and protein levels(P<0.05);Calycosin significantly inhibited the ability of migration and invasion in MDA-MB-231 cells with reduction of VEGF,and these inhibitory effects could be partially suppressed by Rab27 B over-expression.Conclunsion: Calycosin could inhibit the migration and invasion of human breast cancer cells,which may be relevant to the down-regulation of VEGF through inhibition of Rab27 B expression.