The Biological Function Of HtrA1 Serine Protease In Hepatocellular Carcinoma And The Experimental Study On The Tolerance Of Mediated Chemotherapy

Objective: To explore the biological function of HtrA1 in the cells of hepatocellular carcinoma and its relationship with chemotherapy sensitivity.Methods: Obtaining LV-HtrA1-siRNA and negative control LV-NC by Lentivirus infecting.The expression level of HtrA1 in LV-HtrA1-siRNA and LV-NC cells was confirmed by using RT-PCR.The scratch healing test was used to detect changes in cell invasion and migration ability.Using different concentration of adriamycin role respectively in HtrA1 interference of HCC cells and the control group,the CCK 8 were used to detect the cell vitality,flow cytometry analysis technology,Western blotting detect the occurrence of apoptosis.Results: We successfully constructed stable reduced expression of HtrA1 in human gastric cancer cell lines by using RNAi.The cell proliferation assay showed that the proliferation rate of the cell lines with low expression of HtrA1 was significantly lower than the blank transfection group on day 3.The results of CCK-8 proliferation showed that the migration ability of the cell lines with low expression of HtrA1 was significantly enhanced.The results of apoptosis assay showed that the apoptosis cells of HtrA1 were more than that of the blank transfection group.The results of Western blotting showed that the expression of apoptotic inhibitory protein XIAP in HtrA1 low expression group was significantly higher than that in the blank control group,while the apoptotic protein caspase-3 was significantly lower in HtrA1 low expression group than in the blank control group.The results of CCK-8 apoptosis showed that the survival rate of HtrA1 low expression was significantly higher than that of the blank control group under the effect of different concentrations of adriamycin.All of differences were statistically significant.Conclusion: HtrA1 down-regulated expression can significantly enhance the proliferation,migration and invasion ability of HepG2 and Huh7 cells,suggesting that HtrA1 is involved in regulating the biological function of hepatocellular carcinoma cells and is closely related to tumor invasion and metastasis;After the HtrA1 expression was down-regulated,the killing effect of hepatocellular carcinoma cells on adriamycin was enhanced,suggesting that HtrA1 targeted therapy combined with adriamycin could be a new strategy for the treatment of hepatocellular carcinoma.