The Expression Of Vasoactive Intestinal Peptide And Its Relationship With Macrophage Polarization In Hepatocellular Carcinoma

Objective To research the expression of vasoactive intestinal peptide(VIP)and its relationship with macrophage polarization in hepatocellular carcinoma(HCC).Methods Immunohistochemistry was used to examine the expression of VIP protein in 90 cases of HCC and surrounding healthy tissues;and CD68?CD206?CD11c as the macrophage markers in inflammatory were also detected.Western blot and PCR(q RT-PCR)were used to detect the relative expression of VIP in 20 pairs of frozen liver cancer and surrounding tissues.Results Immunohistochemical staining showed that the positive expression rate of VIP in the the 90 HCC tissues was 80%,which appeared at a high level compared to adjacent tissues(29%).There is a relationship between VIP expression status in liver cancer and gender and age(? 2 =12.63?4.756,P < 0.05),while no significant correlation to the TNM stage,tumor size,and other clinical pathological.The results of Western blot and q RT-PCR showed that the expression of VIP in hepatocellular carcinoma was greater than that in the surrounding healthy tissues(P <0.01).The expression of VIP in HCC tissue was positively correlated with CD68 and CD206,and negatively correlated with CD11 c.Conclusion The expression of VIP in HCC tissues is higher than those in adjacent,and is associated with gender and age of in HCC,which indicate that VIP may play a role in the occurrence and development of HCC,The mechanism is VIP may promoteM2 polarization.