A Study On The Correlation Between UQCRC1 Promoter Methylation And The Risk Of Alzheimer’s Disease

Background and Objectives:Alzheimer’s disease(AD)is a chronic progressive neurodegenerative disorder of the central nervous system,and it is the most common cause of dementia.The main clinical features include Progressive memory disorders,cognitive dysfunction,changes in personality,language disorders and other neuropsychiatric symptoms.As the global population aging,the incidence of AD increased significantly,it has a great financial burden on individuals,carers,families and the community.The major public health problem,as well as the associated health care costs,is of national importance.The pathogenesis of AD is not clear so far,yet to find a clinical diagnosis of AD specific markers.Current diagnostic methods for AD include enzyme-linked immunosorbent sandwich(ELISA),PCR-RFLP,auxiliary examinations include electroencephalography,cerebral nuclear magnetic resonance,single photon emission computed tomography(SPECT),positron emission tomography(PET),neuropsychological and scale tests.However,SPECT and PET detection techniques are expensive,and cerebrospinal fluid examination is an invasive test that can not be understood and accepted by most patients.Compared with cerebrospinal fluid,blood specimen collection is more convenient and convenient.So actively looking for a detection method of peripheral blood in early diagnosis of AD is crucial.Mitochondria have an important role in the survival of neurons.The pathogenesis of early onset of Alzheimer’s disease may be related to mitochondrial dysfunction.Ubiquinol-cytochrome c reductase core protein 1(Uqcrc1)is a subunit encoding mitochondrial respiration complex III(an important part of the respiratory chain).If the UQCRC1 gene is methylated,it may affect the function of mitochondrial respiratory chain,and further may affect the susceptibility of AD.At present,peripheral blood gene methylation detection technology has matured.This article aims to investigate the relationship between the methylation of UQCRC1 gene and AD in order to find out the detection method of early diagnosis of AD in peripheral blood and provide a new therapeutic target for AD.Methods:A total of hospitalized patients admitted to the Neurology memory disorders clinic and the inpatient department of the Department of Neurology,China-Japan Union Hospital of Jilin University from September2015 to December 2016 were selected.At the same time,the non-AD patients who matched with the baseline data of the AD group living in the same area,age,educational level,blood pressure and physical condition were selected as the control group in the physical examination center of China-Japan Union Hospital of Jilin University.A total of 105 people were selected.There were 26 males and 24 females in the AD group,there were26 males and 29 females in the control group.Methylation specific PCR(MSP)was used to detect the methylation status of UQCRC1 genes in peripheral blood of the AD group and the control group.Simultaneously,the correlation between methylation of UQCRC1 gene and the patient’s gender and age were analyzed.Results:In 50 AD cases,32 cases were methylated,the methylation rate was64%and in the control group,14 cases were methylated,and the methylation rate was 25.45%.Statistics analysis showed that difference in the AD group and the control group was statistically significant(χ~2=15.807,P<0.05).According to gender stratification,there was no difference in methylation level of UQCRC1 gene in AD group and control group(P>0.05);According to age stratification,there was difference in methylation level of UQCRC1 genne in AD group(P<0.05).Conclusion:1.The methylation of UQCRC1 is related to the susceptibility of Alzheimer’s disease.2.Detection of UQCRC1 gene methylation may have clinical significance in early diagnosis of Alzheimer’s disease.