The Role Of Insulin-like Growth Factor 2 MRNA Binding Protein 2(IGF2BP2)in The Development Of Lung Cancer

Background:At present,cancer has become the leading threat to the health of mankind.The lung cancer is one of the most common malignant tumors worldwide.For the hidden symptoms and high malignant of initial lung cancer,it is always late when the disease is diagnosed and the morbidity and mortality rates are always at the top.Despite of continuous development of surgery and chemical drugs,the treatment outcome is not satisfied.Early diagnosis and timely treatment become the key to prevent the human's health from lung cancer.With the development of molecular biology,tumor-associated antigen(TAA)has been gradually becoming the hot spot of modern medical attention.Not only could patients be diagnosed in the early stage,but also the TAA can provide the basis of tumor targeted therapy.Insulin-like growth factor 2 mRNA binding protein 2(IGF2BP2)is a kind of cytoplasmic protein,which has been proved having a tight connection with many tumors,such as hepatocellular carcinoma,breast cancer,glioma,esophageal carcinoma,etc.But the relationship of IGF2BP2 and lung cancer is still not clear.Objective:This experiment aims to test the expression level of IGF2BP2 in the lung cancer tissues and study the effects of IGF2BP2 on the biological behavior on lung cancer cells.So that we can explore the association of IGF2BP2 and lung cancer,and to clarify the IGF2BP2's effects on the lung cancer.Methods:We explored and analyzed the IGF2BP2 expression and its clinical value through the organization and cell level.Firstly,we collect the lung cancer tissue samples,from which we extracted the total proteins and RNAs.Then we made an analysis through the Western Blot,RT-PCR technology.On the other hand,we explored the influence of expression difference on the biological characteristics of IGF2BP2,using the Western Blot,RT-PCR and cell transfection technique on the cell level.Results:The result shows that the IGF2BP2 in cancer tissues expresses significantly more than that in para-carcinoma tissues(P<0.01).IGF2BP2 has a significant association with the diameter of tumor and the sex(P<0.01),but there is no statistical association between IGF2BP2 and age and clinical stage(P>0.05).The IGF2BP2 in cancer cells expresses significantly more than that in normal lung cells(P<0.01).Knocking out the IGF2BP2 gene in lung cancer cells could reduce the ability of cell proliferation and migration(P<0.01)Conclusion:IGF2BP2 contributes to proliferation and invasion of the lung cancer cells,and plays an important role in the development of lung cancer,which establishes the scientific basic for the clinical early diagnosis and prognosis of lung cancer.