The Multi-target Mechanism Of Synephrine And Paeoniflorin Exert Antidepressive And Prokinetic Effect Similar To Zhiqiao Shaoyao

Objective:At present,many monoamine antidepressants are slow and have a lot of side effects in the market.To CSS A&P as a clue,we find Ferulic Acid and MH,but still do not know whether there is still A&P role.In this study,we focus on inflammatory pathways and compare antidepressant effect and prokinetic effect of ZS and Synephrine and Paeoniflorin from multiple angles through oxidative stress,KYN,ghrelin and other aspects.We initially elucidate its potential mechanism of A&P and ZS and Synephrine and Paeoniflorin may be potential natural antidepressants.Methods:First,we use FST,TST,OFT,gastrointestinal motility experiment methods to compare the antidepressant effects and prokinetic-like effect of ZS and Synephrine and Paeoniflorin.Second,we use ELISA kits to detect gastrointestinal hormone to compare gastrointestinal motility.Third,in order to compare the anti-inflammatory effects,we use ELISA kits to detect it.Fourth,in order to compare the function on the uric acid pathway in CUMS rat,we use ELISA kits to detect concentrations of IDO and KYN in plasma and hippocampus.Fifth,in order to compare the antioxidant capacity,we use ELISA kits to detect the concentrations of ROS and MDA in hippocampus.In addition,to compare the function on the secretion of monoamine neurotransmitters,we use ELISA kits to detect it.Results:We firstly demonstrate that ZS may achieve antidepressant effects through inflammation,oxidation,and uric acid.Second,compared with Synephrine,Paeoniflorin and ZS can more effectively partially reduce the immobility time of FST experiment,inflammatory factors,and significantly improve 5-HT.Third,compared with fluoxetine,ZS and Synephrine and Paeoniflorin can improve the level of gastrointestinal motility hormones in the gastric antrum tissue and plasma,and effectively facilitate gastrointestinal motility.