Pygo2 Affects The Adipogenesis Of Mice By Wnt Signaling Pathway

More and more studies showed thatWnt signaling pathway plays a significant role in regulation of cell proliferation and differentiation.As an energy storage organ,fat tissue' s development and progression is bound up with human health.However,with the improvement of human living standards,they have more opportunities to obtain energy but lower chance to take them into sport,Hence,the number of obese people are on the rise worldwide.Besides,fat tissue is not only as an organ for energy storage,but also plays an important role in secreting many kinds of hormones or hormone-like peptides.So if there are something abnormal occur in differentiation and regulation of adipocyte that will company with all kinds of disease in human body,such as hyperlipidemia,diabetes,fattyliver,and breastcancer.At present,a large number of studies have shown that Wnt signaling pathway in the growth and development of adipose tissue plays an important role in its regulation,but Pygopus2 as an important transcription factor in the Wnt signaling pathway,its regulation of fat formation is still in the blank.In this study,we found that,in vitro,Pygo2 deficient mouse embryonic fibroblasts(MEFs)occurred self-differentiation after contact suppression and become mature adipocyte.According to this phenomenon,we are interested in whether Pygo2 is involved in regulating fat formation.Since Pygo2 systemic elimination of mouse is embryos lethal,we knocked out Pygo2 in adipose tissue conditionallyby using aP2-cre,Pygo2flox/flow mouse.By measuring the weight,histological sections,HE staining,and other method,we found hypertrophy occur in Pygo2 deficient mice fat tissue.Through extracting mice fat tissue RNA and measuring its fat-relative marker protein by real-time PCR,we found,compare to wildtype mice,the content of this protein is increased significantly in Pygo2 deficient mice fat tissue.At the cellular level,we found that knockdown Pygo2,by using RNA interference,can promote 3T3-L1 pre-adipocytes,and inhibitits differentiation by overexpression of Pygo2.