Mass Spectrometry Analysis Of Gab2 Binding Proteins In HepG2 Cell

Fatty liver,the most common cause of chronic liver disease and metabolic disorders,has seriously affected global human health.Fatty liver from the beginning of steatosis,steatohepatitis,cirrhosis,hepatocellular carcinoma and a variety of extrahepatic complications(cardiovascular disease,other organs of malignant tumors).Gab2 acts as a platform signaling protein that mediates complex protein-protein interactions.Previous studies confirmed that oleic acid and alcohol induced an increase in Gab2 expression and promoted Gab2 to raise PI3K,Shp2 or Socs3 and activate their downstream signals such as Akt,Erk and Stat3.Therefore,Gab2 is an important liver protein associated protein,is a causative agent induced fatty liver signal integration hub.But people on Gab2 specifically how to integrate a variety of signals to control fatty liver is still little known.In this study,three experiments were carried out on qualitative mass spectrometry based on affinity purification.621,780,537 proteins were identified respectively.There were 408 proteins in these proteins containing 23 known interacting proteins,and 385 newly discovered Gab2 Binding protein.These proteins were involved in cell cycle regulation,cell localization,transmembrane receptor tyrosine kinase signaling,and so on,and were involved in the study of glycerol phospholipids by means of STRING and Metascape software.Biosynthesis,lactic acid synthesis and metabolism,epithelial cell signaling,etc.These metabolic signals are closely related to the accumulation of fat and the development of fatty liver,suggesting that Gab2 as a platform protein,does mediate a large number of related proteins involved in the regulation of fatty liver Come in.The subject also utilizes multidimensional mass spectrometry and cell stabilization isotope amino acid labeling techniques to systematically analyze and reveal the dynamic quantitative changes of the important proteins associated with refueling 2 oleic acid-induced quantitative mass spectrometry experiments identified 153 proteins,of which an increase of 2 More than 15 times the protein and alcohol-induced quantitative mass spectrometry experiments identified 175 proteins,of which more than 2 times the increase in protein 20.Proteins-protein interaction analysis of these proteins by String software showed that these proteins had a direct interaction with the Gab2 proteinIn this paper,the interaction network of Gab2-binding protein was established by using mass spectrometry-based proteomics for the first time.The dynamic interaction of Gab2 protein in the fatty liver model was revealed,which was expected to be the prevention of fatty liver And the development of new drugs to provide an important scientific basis.