TRAP1 Expression And Its Influencing Factors In Hepatocellular Carcinoma

Objective: By comparing hepG2 and stable transfection HBV hepG2(hep G2.2.15),different invasion degree of HCC cell lines(low invasive in hep3B2.1-7,aggressive in hepG2 and high invasive in sk-hep1)and when they are in different level of autophagy by rapamycin induced the Beclin1,LC3-?/LC3-?and TRAP1 expression level,to evaluate the relationship and effect of the three different factors on the level of autophagy and TRAP1 expression level;Through comparing the cell growth curve of the different invasive HCC cell lines,hepG2 and stable transfection HBV hepG2(hepG2.2.15)and the hepG2 in different level of autophagy,discusses the influence of three different factors on the cell growth and mutual relations.Objective: By comparing hepG2 and stable transfection HBV hepG2(hep G2.2.15),different invasion degree of HCC cell lines(low invasive in hep3B2.1-7,aggressive in hepG2 and high invasive in sk-hep1)and when they are in different level of autophagy by rapamycin induced the Beclin1,LC3-?/LC3-?and TRAP1 expression level,to evaluate the relationship and effect of the three different factors on the level of autophagy and TRAP1 expression level;Through comparing the cell growth curve of the hepG2 in different level of autophagy,discusses the influence of different level ofautophagy for cell growth.Methods: Differences in TRAP1 geneexpression and autophagy protein Beclin1,LC3-?/LC3-?expression at the protein and mRNA levels using quantitative RT-PCR,protein simple and Western Blotting analysis,and use the MDC fluorescent staining to observe cell autophagy level;Using cell count plate under a microscope for five days counting cell number to determination the cell growth curve.Results:In the different invasive cancer of the liver cell lines,TRAP1 expression level and the level of autophagy was highest in hepG2,hep3B2.1-7 is higher,and the lowest in sk-hep1;Cell autophagy level and TRAP1 expression level in transfection of HBV hep G2(hepG2.2.15)was lower than that in the not transfection HBV hepG2;With the increase of concentration of rapamycin,cell autophagy level increases,the top10 ?mol/L,TRAP1 expression level in the same change trend in hep G2,hep3B2.1-7 and sk-hep1,but in hepG2.2.15,with an increased level of autophagy,TRAP1 expression level lower instead.In hepG2,with the increase of concentration of rapamycin,cell growth speed increase,10 ?mol/L,the fastest and 15 mu mol/L instead of slowing down.Conclusions: Different invasive HCC cell lines,cell autophagy level and TRAP1 expression level are different;HBV inhibition of cell autophagy and the expression of TRAP1.Rapamycin induced cell autophagy promotes the expression of TRAP1,and HBV inhibit rapamycin induced cell autophagy promotes the expression of TRAP1;Appropriate range increase cell autophagy level can promote cell growth,and high level of autophagy inhibition of cell growth.