The Effect Of Human IPS Cells On Epithelial Mesenchymal Transition In Human Lung Adenocarcinoma A549 Cells

Induced pluripotent stem cells(iPSCs)are associated with the advantages of multi-lineage differentiation and strong self-proliferation.Stem cell therapy is an important treatment that is applied in numerous fields such as experiment or clinical medicine.However,the interaction between stem cells and tumor cells remains unknown.It is indicated in some research that stem cells can promote growth and metastasis of tumor.However,contrary results have also been reported in some studies,which suggest that stem cells inhibit tumor growth.Therefore,this paper focuses on the effect of human iPS cells in the occurrence of epithelial-mesenchymal transition in human lung adenocarcinoma A549 cells.Research objective:The effect of human iPS cells on epithelial-mesenchymal transition in human lung adenocarcinoma A549 cells.Research methods:Optimized culture conditions of human iPS cells and human lung adenocarcinoma A549 cells,so that cells had excellent proliferation and growth status.Indirect contact co-culture of human iPS cells and human lung adenocarcinoma A549 cells was carried out using Transwell chamber,cytokines secreted by human iPS cells could permeate into the microenvironment of human lung adenocarcinoma A549 cells through the filter membrane.Research under the conditions of indirect contact co-culture of human iPS cells,changes in morphology,invasion ability,apoptosis and proliferation of human non-small cell lung adenocarcinoma A549 cells,as well as in expression of EMT-related genes vimentin,E-cadherin,N-cadherin,Snail,Slug,Twist,MMP-2 and MMP-9 were studied.Research resultsand conclusion:(1)Compared with blank control group and the negative control group,experimental group human iPS cells with human lung adenocarcinoma A549 cells after co-culture,the cellular morphology of human lung adenocarcinoma A549 cells showed mesenchymal-like changes,enhance the invasion ability,reduce the apoptosis rate,improve the proliferation ability,The mRNA expression quantity of MMP-9 in experiment group had increased by 6.06 folds compared with that in negative control group,while 5.49 folds relative to that in blank control group,and that had significantly increased.The mRNA expression quantities of vimentin increased,mRNA expression quantity of E-cadherin slightly reduced,mRNA expression of the other genes,N-cadherin,Slug,Snail,Twist,MMP-2 the differences were not significant.(2)The results show that human iPS cells with human lung adenocarcinoma A549 cells after co-culture can promote epithelial-mesenchymal transition in human lung adenocarcinoma A549 cells,and enhance the migration and invasion abilities of human lung adenocarcinoma A549 cells.