Research Of Magnetic Targeting Photodynamic Multifunctional Nano-delivery System In Visible Treatment Of Breast Cancer

In the world,breast cancer is one of the most common and most likely lethal malignant tumors in women.Paclitaxel(PTX)is a widely used broad-spectrum anti-tumor drugs and is also one of the first choice for clinical treatment of breast cancer.However,due to the low water solubility of paclitaxel,and the way for the treatment of breast cancer is single,the clinical use of paclitaxel is limited.The development of new formulations of paclitaxel has continued to receive widespread attention at home and abroad over the years.It is an effective means to realize the key problems in breast cancer imaging and synchronous therapy by developing nano-materials with light/magnetic effect and new technical means to develop nano-paclitaxel with visual response and specific targeting for breast cancer.The aim of this study was to develop a multifunctional paclitaxel nanocomposite with good clinical adaptability,magnetic targeting and transferrin ligand targeting,as well as photodynamic therapy.In this paper,water-soluble Fe3O4 nanoparticles with superparamagnetism were prepared by hydrothermal coprecipitation method.Liposomal nano-drug containing paclitaxel and Fe3O4 magnetic nanoparticles were prepared by thin film dispersion method.Block copolymers could stabilize the structure of liposomal nano drugs and conjugate transferrin-tumor targeting ligand.By modifying the block copolymer on liposomal surface,the nano-drug has the effect of tumor ligand targeting and magnetic targeting therapy,while at the same time 5-aminolevulinic acid(5-aminolevulinic acid,5-ALA),a kind of photodynamic therapy drug,was included in the liposomal nano drugs to synchronize the active targeted photodynamic therapy of the tumor.The results showed that the average particle size of paclitaxel magnetic liposomes nano-drug was lower than 200nm with negative zeta potential,which is beneficial to the long circulation of the carrier drug in the body;The dispersibility of nano-drug in the aqueous solution was good,the high entrapment efficiency of paclitaxel in liposomes was 89.92%and the 11.27%entrapment efficiency of 5-ALA was also higher than that of previous reports 5.7%;In the case of cytotoxicity test,dual-drug magnetic nano-drug showed significant killing of breast cancer cells,and the 5-ALA photo sensitizer contained in the nano-drug was used for photodynamic therapy with obvious effects;The coupling rate of transferrotein was 35.6%,which had significant differences in the killing of breast cancer cells,indicating that nano-drug have a certain degree of active targeting;In vivo test,the paclitaxel magnetic liposomes exhibited long cycle and magnetic targeting properties,while nano-drug containing superparamagnetic Fe3O4 can be used for in vivo MRI visualization tracer.The results of the above studies suggest that the magnetic targeting photodynamic multifunctional nano-delivery system in this paper can improve the clinical application value of paclitaxel,it has the potential to achieve the active chemotherapy of tumor and active targeted photodynamic therapy simultaneously,which can achieve a variety of treatment of tumor synchronization precision treatment.This context has a certain reference value for "multi-modal imaging" research.