| Objective:To explore the pathogenesis of malignant ovarian cancer with endometriosis,predict the risk of EM malignancy and find the basis for targeted therapy,we analyze the different expression of the two indicators in each groupand the correlation between ER-β and clinical indicators by examining the expression intensity of normal ovarian,endometriosis(EM),endometriosis associated with ovarian cancer(EAOC),estrogen receptor beta(estrogen Receptorp)in other epithelial ovarian cancer and tyrosine kinase receptor type B(TrkB)·Method:10 cases of ovarian tissue which accepting by surgical treatment in January 2010-December 2014 at Dalian Maternal and Child Health Hospital were collected as the normal group.10 case of postoperative pathology results showing ovarian endometriosis which were treated by surgical were collected as the EM group.18 cases of ovarian tissue whose postoperative pathological results showing endometriosis and cancer coexist in the same ovary were selected.The age,length of disease,menopause,tumor size,CA-125 value,histological grade and FIGO staging were calculated for each patients.The expression of ER-p and TrkB was detected by immunohistochemical SP method.Image-pro-plus 6.0 software was used to analyze the expression intensity of each index.Calculate the average optical density value.Using Spss21.0 statistical software for data processing.P<0.05 indicating that the difference was statistically significant.Results:1.The expression of ER-β in the control group and the experimental group:The expression of ER-p in EM group was higher than that in normal group.The expression of ER-β in EAOC group was lower than that in normal group.The differences in the intensity of expression between groups were statistically significant(P<0.05).There was no significant difference of ER-β expression between endometrioid adenocarcinoma and clear cell carcinoma(P>0.05)·2.The expression of TrkB in the control group and the experimental group:The expression of TrkB in EM group was higher than that in normal group.While the expression of TrkB in EAOC was higher than that in endometriosis group.The differences in the intensity of expression between groups were statistically significant(P<0.05).There was no significant difference of the expression of TrkB between endometrioid adenocarcinoma and clear cell carcinoma(P>0.05)3.The relativity of ER-B and TrkB in the control group:There was a positive correlation between the expression of ER-β and the expression of TrkB in EM.The correlation coefficient was 0.782(P<0.05).In EAOC,the expression of ER-β was negatively correlated with the expression of TrkB.The correlation coefficient is-0.765(P<0.05).4.The correlation between expression intensity of ER-B and some clinical indicators:The expression intensity of ER-B was negatively correlated with the FIGO stage in EAOC.The correlation coefficient is-0.020(P<0.05);The expression intensity of ER-β was not correlated with age,length of disease,CA-125 value,tumor size,menopause and differentiation.Conclusion:1.High expression of ER-β in EM which increase endometrial adhesion and proliferation induced the occurrence of endometriosis.ER-β is mainly expressed as tumor suppressor gene in EAOC.Its expression was significantly reduced,which inhibit the ability of apoptosis and mediate ovarian cancer.2.The expression intensity of TrkB in EM was higher than that in control group.By participating in the inhibition of ectopic endometrial cell apoptosis as well as regulating cell migration.it induced the occurrence of EM.However,The expression intensity of TrkB in EAOC was higher than that in EM group.By activating the P13K/Akt pathway,PLC pathway,and increased Ca2+ release,highly expressed TrkB act on the downstream signaling pathways,thereby regulating cell growth.3.The expression of ER-p in EM was positively correlated with the expression of TrkB in EM.E2/ER-β pathway could directly activate and up-regulate the expression of TrkB through the non-nuclear effect of estrogen.Finally it enhance cell anti-apoptotic ability through the PI3K signal pathway,leading to the occurrence of EM.There was a negative correlation between ER-β and TrkB expression in EAOC,which may be related to the decrease of ER-β variant/wild type ratio and the enhancement of TrkB expression in EAOC.The specific mechanism remains to be further studied.4.The expression intensity of ER-β was negatively correlated with FIGO staging in EAOC,which may be related to its antiproliferative effect.There is no significantly correlation among expression intensity of ER-β with age,length of disease,menopause,CA-125,tumor size and tumor classification. |
