The Study Of FOXG1 To Regulate Apoptosis In Gliomas

Gliomas are one of the most common primary tumors in the central nervous system,which is 30% of total tumors in brain.The common clinical treatments include surgery,radiotherapy and chemotherapy.Although much effort has been applied to treat gliomas,the prognosis is still poor and recurrence rate is high.According to WHO classification system,gliomas are classified into four different grades,grade I(pilocytic astrocytoma)and grade II(diffuse astrocytoma);and grade III(anaplastic astrocytoma)and grade IV(glioblastoma multiforme;GBM).FOXG1 is a member of the forkhead box protein family,which is essential in the development of mammalian telencephalon.There are some reports also showed that FOXG1 play roles in the development of some cancers,such as hepatoblastoma,medulloblastoma,gliomas,ovarian cancer,breast cancer and cervical cancer.Studies of FOXG1 in gliomas have shown that FOXG1 is highly expressed in grade II,grade III and grade IV gliomas,and is associated with the poor prognosis in these malignant gliomas.However,there is still very little knowledge about how FOXG1 regulate apoptosis in gliomas.In this thesis,we investigated the roles of FOXG1 in glioma cell proliferation and apoptosis.Firstly,we collected fresh glioma samples from clinical patients and examined the expression level of FOXG1 in glioma tissues and the peripheral tissues using western blotting analysis.We found the expression level of FOXG1 are higher in glioma tissuess than that in glioma peripheral tissues in about two thirds of our glioma samples.This result was also confirmed in our immunohistochemistry analysis.We also found the expression of FOXG1 was elevated with the increase of glioma malignancy.In addition,we also analyzed the correlation of FOXG1 with other clinical factors.The statistics analyses showed that FOXG1 expression was only associated with glioma malignancy but not any other clinical parameters,such as age,gender,acute or chronic of diseases,and the time length in the hospital.We can confirmed that the expression level of FOXG1 is increased with the glioma malignancy.To further study the mechanism of FOXG1,we detected the expression levels of FOXG1 in U87 MG,U251 and SHG44 glioma cells lines and according to the result,we then used lentivirus systems to knock down and overexpress FOXG1 in glioma cells.Afterwards,we detected the proliferation and apoptosis in glioma cells.Results showed FOXG1 can promote glioma proliferation,and inhibit apoptosis and the expression of some Caspase proteins.In summary,our study revealed the fact that the expression level of FOXG1 in gliomas increased with the degree of glioma malignancy,and also confirmed the fact that FOXG1 canpromote glioma proliferation and inhibit the expression of some of Caspase proteins.These results may let FOXG1 to be a new potential drug targets to treat gliomas.