The Preliminary Research Of Human Peripheral Blood Mo-MDSCs In The Diagnosis Of Breast Cancer

Myeloid-derived suppressor cells(MDSCs)are heterogeneous cell groups which are well known for its suppression of T-cell responses.Researches showed that MDSCs are not present in healthy individuals when they are in the steady state,they just appear in some pathologic conditions,like cancer,infection and so on.Ample evidences support that MDSCs play a key role in immune suppression in cancer.They also promote the process of tumor angiogenesis,tumor metastasis and drug resistance.Accordingly,targeting MDSCs may represent an attractive diagnostic and therapeutic opportunity.According to the differences of morphology and phenotype,MDSCs consist of two large groups of cells termed granulocytic or polymorphonuclear MDSCs(PMN-MDSCs)and monocytic MDSCs(mo-MDSCs).Researches suggests that mo-MDSCs have stronger capability in suppression in terms of a single cell.The objective of the study was to explore and evaluate the applicability of monocytic MDSCs(mo-MDSCs)in diagnosing breast cancer(BC).In this study,we analyzed the percentage of mo-MDSCs in the freshly isolated peripheral blood of patients with BC or breast benign tumor(BT)and healthy donors by flow cytometry.We found it was 2.60% ± 0.95% in healthy donors,2.94% ± 0.82% in BT patients and 3.89% ± 1.35% in BC patients.The percentage of circulating mo-MDSCs increased significantly in BC patients and it was positively correlated with the clinical stage of BC patients.Receiver operating characteristic curve analysis showed 80.5% sensitivity,61.3% specificity and a 76.6% AUC which meant a medium clinical application value of mo-MDSCs in the diagnosis of BC.Our results suggested that the percentage of circulating mo-MDSCs in human peripheral blood may be a potential indicator in the auxiliary diagnosis of BC.